Literature DB >> 6662892

Rapid quantitation of ultraviolet-induced thymine-containing dimers in human cell DNA by reversed-phase high-performance liquid chromatography.

J Cadet, N E Gentner, B Rozga, M C Paterson.   

Abstract

Rapid and efficient separation of all three types of cyclobutyl pyrimidine dimer (Pyr mean value of Pyr) species induced in cellular DNA by far-ultraviolet (UV) light (chiefly 254 nm) has been achieved by reversed-phase high-performance liquid chromatography using octadecylsilyl stationary phases. The order of elution is: (Ura mean value of Ura) less than (Ura mean value of Thy) less than (Thy mean value of Thy) less than Thy. The determination of Pyr mean value of Pyr species in DNA from UV-irradiated, [3H]thymidine-labelled human skin fibroblasts in tissue culture is demonstrated for far-UV fluences as low as 10 J/m2. The ability to measure specifically individual dimer types allowed demonstration of comparable kinetics of repair for two labelled dimer species (Ura mean value of Thy and Thy mean value of Thy).

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Year:  1983        PMID： 6662892     DOI： 10.1016/s0021-9673(00)91543-7

Source DB:  PubMed          Journal:  J Chromatogr


  9 in total

1.  Determination of photomodified oligodeoxynucleotides by exonuclease digestion, matrix-assisted laser desorption/ionization and post-source decay mass spectrometry.

Authors:  L K Zhang; Y Ren; D Rempel; J S Taylor; M L Gross
Journal:  J Am Soc Mass Spectrom       Date:  2001-10       Impact factor: 3.109

2.  UV-absorbing substance in the red alga Porphyra yezoensis (Bangiales, Rhodophyta) block thymine photodimer production.

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Journal:  Mar Biotechnol (NY)       Date:  2003 Mar-Apr       Impact factor: 3.619

3.  Inter-strand photoproducts are produced in high yield within A-DNA exposed to UVC radiation.

Authors:  Thierry Douki; Grégory Laporte; Jean Cadet
Journal:  Nucleic Acids Res       Date:  2003-06-15       Impact factor: 16.971

Review 4.  Investigating the biochemical impact of DNA damage with structure-based probes: abasic sites, photodimers, alkylation adducts, and oxidative lesions.

Authors:  Heidi A Dahlmann; V G Vaidyanathan; Shana J Sturla
Journal:  Biochemistry       Date:  2009-10-13       Impact factor: 3.162

5.  Purification and characterization of a novel human acidic nuclease/intra-cyclobutyl-pyrimidine-dimer-DNA phosphodiesterase.

Authors:  K S Famulski; M Liuzzi; S Bashir; R Mirzayans; M C Paterson
Journal:  Biochem J       Date:  2000-02-01       Impact factor: 3.857

6.  Essential role of ultraviolet radiation in the decrease of corneal endothelial cell density caused by pterygium.

Authors:  Xia Li; Yiqin Dai; Weiwei Xu; Jianjiang Xu
Journal:  Eye (Lond)       Date:  2018-08-29       Impact factor: 3.775

Review 7.  Nrf2, a Potential Therapeutic Target against Oxidative Stress in Corneal Diseases.

Authors:  Xiu-Fen Liu; Dan-Dan Zhou; Tian Xie; Tayyab Hamid Malik; Cheng-Bo Lu; Hai-Jun Li; Fan Wang; Chang Shu; Cong Liu; Cheng-Wei Lu; Ji-Long Hao
Journal:  Oxid Med Cell Longev       Date:  2017-10-25       Impact factor: 6.543

Review 8.  Fuchs endothelial corneal dystrophy: The vicious cycle of Fuchs pathogenesis.

Authors:  Stephan Ong Tone; Viridiana Kocaba; Myriam Böhm; Adam Wylegala; Tomas L White; Ula V Jurkunas
Journal:  Prog Retin Eye Res       Date:  2020-05-08       Impact factor: 21.198

9.  UV-A Irradiation Activates Nrf2-Regulated Antioxidant Defense and Induces p53/Caspase3-Dependent Apoptosis in Corneal Endothelial Cells.

Authors:  Cailing Liu; Dijana Vojnovic; Irene E Kochevar; Ula V Jurkunas
Journal:  Invest Ophthalmol Vis Sci       Date:  2016-04-01       Impact factor: 4.799
  9 in total

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