Literature DB >> 6661195

The biosynthesis of dentine gamma-carboxyglutamic acid-containing proteins by rat incisor odontoblasts in organ culture.

M T DiMuzio, M Bhown, W T Butler.   

Abstract

Rat odontoblasts were shown to synthesize and secrete gamma-carboxyglutamic acid(Gla)-containing proteins into dentine after organ culture in the presence of radiolabelled amino acid precursors. Purified dentine Gla-containing protein from rat incisors was used as antigen to prepare rabbit antisera as a probe of dentine Gla-containing-protein biosynthesis in organ cultures of dentine (rat incisor) and bone (rat calvaria). Use of the antiserum also pointed out the cross-reactivity of a high-M, glycoprotein present within the dentine matrix. The present results are significant in identifying dentine gla-containing protein as endogenous to mineralizing dentine and may relate to the commonality between calcifying connective tissues in general.

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Year:  1983        PMID： 6661195      PMCID： PMC1152499          DOI： 10.1042/bj2160249

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  27 in total

1.  Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors:  S W Kessler
Journal:  J Immunol       Date:  1975-12       Impact factor: 5.422

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3.  Isolation and partial characterization of a glycoprotein from bovine cortical bone.

Authors:  B A Ashton; J T Triffitt; G M Herring
Journal:  Eur J Biochem       Date:  1974-06-15

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Journal:  Bull World Health Organ       Date:  1974       Impact factor: 9.408

5.  A method of trace iodination of proteins for immunologic studies.

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6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  An improved fluorometric assay for DNA.

Authors:  R T Hinegardner
Journal:  Anal Biochem       Date:  1971-01       Impact factor: 3.365

8.  Biosynthesis of phosphoprotein by rat incisor odontoblasts in in-vitro culture.

Authors:  E C Munksgaard; W S Richardson; W T Butler
Journal:  Arch Oral Biol       Date:  1978       Impact factor: 2.633

9.  Vitamin K dependence of a calcium-binding protein containing gamma-carboxyglutamic acid in chicken bone.

Authors:  P V Hauschka; M L Reid
Journal:  J Biol Chem       Date:  1978-12-25       Impact factor: 5.157

10.  Direct identification of the calcium-binding amino acid, gamma-carboxyglutamate, in mineralized tissue.

Authors:  P V Hauschka; J B Lian; P M Gallop
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205
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  8 in total

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2.  Localization and synthesis of type III collagen and fibronectin in human reparative dentine. Immunoperoxidase and immunogold staining.

Authors:  H Magloire; A Joffre; D J Hartmann
Journal:  Histochemistry       Date:  1988

3.  Immunocytochemical localization of gamma-carboxyglutamic acid-containing proteins (osteocalcin) in rat bone and dentin.

Authors:  A J Camarda; W T Butler; R D Finkelman; A Nanci
Journal:  Calcif Tissue Int       Date:  1987-06       Impact factor: 4.333

4.  Sialylation of terminal saccharides of glycoconjugates expressed by murine molar tooth germs developing in vitro and in vivo.

Authors:  A K Jowett; S J Kimber; M W Ferguson
Journal:  J Anat       Date:  1994-08       Impact factor: 2.610

5.  Ultrastructural localization of osteocalcin in rat tooth germs by immunogold staining.

Authors:  I Gorter de Vries; D Coomans; E Wisse
Journal:  Histochemistry       Date:  1988

6.  Differential gene expression of bgp and mgp in trabecular and compact bone of Atlantic salmon (Salmo salar L.) vertebrae.

Authors:  Christel Krossøy; Robin Ornsrud; Anna Wargelius
Journal:  J Anat       Date:  2009-10-06       Impact factor: 2.610

7.  The biosynthesis of dentin phosphophoryns by rat incisor odontoblasts in organ culture.

Authors:  M T DiMuzio; M Bhown; W T Butler
Journal:  Calcif Tissue Int       Date:  1985-05       Impact factor: 4.333

8.  Vitamin K-dependent gamma-glutamylcarboxylase in Atlantic salmon (Salmo salar L.).

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  8 in total

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