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Literature DB >> 6660518

Determination of the specific activity of NaB3H4.

Abstract

A method for the rapid determination of the specific activity of NaB3H4 is presented. NaB3H4 is used to reduce NAD+ to [3H]NADH, which is then isolated by anion exchange chromatography. The specific activity of the NaB3H4 is calculated from measurements of radioactivity and absorbance (340 nm) in the [3H]NADH fractions.

Entities: Chemical

Mesh：

Substances：

Year: 1983 PMID： 6660518 DOI： 10.1016/0003-2697(83)90709-1

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

1 in total

1. Synthesis of [4S-² H]NADH, [4R-² H]NADH, [4-² H₂ ]NADH and [4-² H]NAD⁺ cofactors through heterogeneous biocatalysis in heavy water.

Authors: Jack S Rowbotham; Adam P Hardy; Holly A Reeve; Kylie A Vincent
Journal: J Labelled Comp Radiopharm Date: 2021-01-26 Impact factor: 1.921

1 in total

Determination of the specific activity of NaB3H4.

1. Synthesis of [4S-2 H]NADH, [4R-2 H]NADH, [4-2 H2 ]NADH and [4-2 H]NAD+ cofactors through heterogeneous biocatalysis in heavy water.

1. Synthesis of [4S-² H]NADH, [4R-² H]NADH, [4-² H₂ ]NADH and [4-² H]NAD⁺ cofactors through heterogeneous biocatalysis in heavy water.