Literature DB >> 6660491

Purification and separation of tomato isolectins by chromatofocusing.

D C Kilpatrick, J Weston, S J Urbaniak.   

Abstract

Tomato lectin can be rapidly separated from a crude extract of tomato fruit proteins by chromatofocusing. The lectin is recovered from a PBE94 column in two peaks, each with a specific activity comparable to that of lectin purified by affinity chromatography on ovomucoid-Sepharose. Both isolectins consist of a single polypeptide chain (Mr 68,000) and have similar properties.

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Year:  1983        PMID： 6660491     DOI： 10.1016/0003-2697(83)90285-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Lectin conjugates as biospecific contrast agents for MRI. Coupling of Lycopersicon esculentum agglutinin to linear water-soluble DTPA-loaded oligomers.

Authors:  Irena Pashkunova-Martic; Christian Kremser; Markus Galanski; Petra Schluga; Vladimir Arion; Paul Debbage; Werner Jaschke; Bernhard Keppler
Journal:  Mol Imaging Biol       Date:  2011-06       Impact factor: 3.488

2.  A comparison of tomato (Lycopersicon esculentum) lectin with its deglycosylated derivative.

Authors:  D C Kilpatrick; C Graham; S J Urbaniak; C E Jeffree; A K Allen
Journal:  Biochem J       Date:  1984-06-15       Impact factor: 3.857

3.  The tomato lectin consists of two homologous chitin-binding modules separated by an extensin-like linker.

Authors:  Willy J Peumans; Pierre Rougé; Els J M Van Damme
Journal:  Biochem J       Date:  2003-12-15       Impact factor: 3.857

4.  A comparison of the lectin-binding properties of glycoconjugates from a range of Leishmania species.

Authors:  R J Rossell; A F Stevens; M A Miles; A K Allen
Journal:  Parasitol Res       Date:  1990       Impact factor: 2.289

5.  Bioadhesion by means of specific binding of tomato lectin.

Authors:  C M Lehr; J A Bouwstra; W Kok; A B Noach; A G de Boer; H E Junginger
Journal:  Pharm Res       Date:  1992-04       Impact factor: 4.200

6.  Design, synthesis, physical and chemical characterisation, and biological interactions of lectin-targeted latex nanoparticles bearing Gd-DTPA chelates: an exploration of magnetic resonance molecular imaging (MRMI).

Authors:  Irena Paschkunova-Martic; Christian Kremser; Klaudia Mistlberger; Nadezhda Shcherbakova; Hermann Dietrich; Heribert Talasz; Yiping Zou; Beate Hugl; Mathea Sophia Galanski; Elisabeth Sölder; Kristian Pfaller; Isabella Höliner; Wolfgang Buchberger; Bernhard Keppler; Paul Debbage
Journal:  Histochem Cell Biol       Date:  2005-04-12       Impact factor: 2.531
  6 in total

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