Preparation of crystalline bovine liver beta-glucuronidase.

A method is described for the preparation of pure beta-glucuronidase from bovine liver. The procedure includes ammonium sulfate, acetone and ethanol fractionation and a simple two-step ion exchange chromatography. The yield is acceptable and the method requires only standard laboratory equipment. The pure enzyme is easily crystallized from ammonium sulfate. Some practical applications of the pure beta-glucuronidase are discussed.