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Literature DB >> 6646117

Co-purification of coenzyme-dependent enzymes by affinity chromatography.

Abstract

Recent developments in general ligand affinity chromatography have greatly facilitated the purification of numerous coenzyme-dependent enzymes in practical scale. By immobilizing various derivatives of coenzymes (NAD+, NADP+, ATP and coenzyme A) on Sepharose, these general ligand affinity columns have the potential of enriching as many as 30% of the two thousand known enzymes as well as their isozymic forms, if they are applied in combination with the conventional procedures.

Entities: Chemical

Mesh：

Substances：

Year: 1983 PMID： 6646117 DOI： 10.1007/BF00223522

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

46 in total

1. Purification of cofactor-dependent enzymes by affinity chromatography.

Authors:
Journal: Anal Biochem Date: 1977-01 Impact factor: 3.365

2. LIVER ALCOHOL DEHYDROGENASE-DPN-PYRAZOLE COMPLEX: A MODEL OF A TERNARY INTERMEDIATE IN THE ENZYME REACTION.

Authors: H THEORELL; T YONETANI
Journal: Biochem Z Date: 1963

3. Characteristics of 8-substituted adenine nucleotide derivatives utilized in affinity chromatography.

Authors: N O Kaplan
Journal: Arch Biochem Biophys Date: 1975-06 Impact factor: 4.013

4. Purification of kinases by general ligand affinity chromatography.

Authors: C Y Lee; L H Lazarus; D S Kabakoff; P J Russell; M Laver; N O Kaplan
Journal: Arch Biochem Biophys Date: 1977-01-15 Impact factor: 4.013

5. Purification of nicotinamide-adenine dinucleotide-dependent dehydrogenases by affinity chromatography.

Authors: K Mosbach; H Guilford; P O Larsson; R Ohlsson; M Scott
Journal: Biochem J Date: 1971-11 Impact factor: 3.857

6. The synthesis of adenine-substituted derivatives of NADP and their potential as active coenzymes and affinity adsorbents.

Authors: C R Lowe; K Mosbach
Journal: Eur J Biochem Date: 1974-12-02

Co-purification of coenzyme-dependent enzymes by affinity chromatography.

1. Purification of cofactor-dependent enzymes by affinity chromatography.

2. LIVER ALCOHOL DEHYDROGENASE-DPN-PYRAZOLE COMPLEX: A MODEL OF A TERNARY INTERMEDIATE IN THE ENZYME REACTION.

3. Characteristics of 8-substituted adenine nucleotide derivatives utilized in affinity chromatography.

4. Purification of kinases by general ligand affinity chromatography.

5. Purification of nicotinamide-adenine dinucleotide-dependent dehydrogenases by affinity chromatography.

6. The synthesis of adenine-substituted derivatives of NADP and their potential as active coenzymes and affinity adsorbents.

7. Cytoplasmic malic enzyme from mouse kidneys.

8. Purification of alcohol dehydrogenase from Drosophila by general-ligand affinity chromatography.

9. Multiple forms of Drosophila hexokinase. Purification, biochemical and immunological characterization.

10. Preparation of adenosine nucleotide derivatives suitable for affinity chromatography.

1. Affinity separation. Patents and literature.