Literature DB >> 6641744

Non-nucleosomal configuration of chromatin in nucleolar organizer regions of metaphase chromosomes in situ.

D Hernandez-Verdun, M Derenzini.   

Abstract

The structure of metaphase chromatin in a human tumor cell line, TG cells, was investigated using thin sections selectively stained for DNA with the Feulgen-like osmium-ammine reaction. The bulk of metaphase chromatin was characterized by the nucleosomal configuration. Some specimens were pretreated by silver staining for selective visualization of acidic proteins of the nucleolar organizer regions. In these specimens, the osmium-amine DNA tracer revealed that the chromatin present at the sites of silver granule localization had a completely extended configuration, and never gave rise to nucleosomal structures.

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Year:  1983        PMID： 6641744

Source DB:  PubMed          Journal:  Eur J Cell Biol        ISSN： 0171-9335            Impact factor:   4.492


  9 in total

1.  Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level.

Authors:  D Ploton; M Menager; P Jeannesson; G Himber; F Pigeon; J J Adnet
Journal:  Histochem J       Date:  1986-01

2.  Three-dimensional organization of the ribosomal genes and Ag-NOR proteins during interphase and mitosis in PtK1 cells studied by confocal microscopy.

Authors:  I Robert-Fortel; H R Junéra; G Géraud; D Hernandez-Verdun
Journal:  Chromosoma       Date:  1993-02       Impact factor: 4.316

3.  Relative distribution of rDNA and proteins of the RNA polymerase I transcription machinery at chromosomal NORs.

Authors:  J A Suja; J Gébrane-Younès; G Géraud; D Hernandez-Verdun
Journal:  Chromosoma       Date:  1997-06       Impact factor: 4.316

4.  Microwave irradiation improvements in the silver staining of the nucleolar organizer (Ag-NOR) technique.

Authors:  F J Medina; A Cerdido; R Marco
Journal:  Histochem Cell Biol       Date:  1995-06       Impact factor: 4.304

5.  Electron tomography of metaphase nucleolar organizer regions: evidence for a twisted-loop organization.

Authors:  L Heliot; H Kaplan; L Lucas; C Klein; A Beorchia; M Doco-Fenzy; M Menager; M Thiry; M F O'Donohue; D Ploton
Journal:  Mol Biol Cell       Date:  1997-11       Impact factor: 4.138

6.  The Ag-NOR proteins and transcription and duplication of ribosomal genes in mammalian cell nucleoli.

Authors:  A Pession; F Farabegoli; D Treré; F Novello; L Montanaro; S Sperti; F Rambelli; M Derenzini
Journal:  Chromosoma       Date:  1991-05       Impact factor: 4.316

7.  Structure of ribosomal genes of mammalian cells in situ.

Authors:  M Derenzini; D Hernandez-Verdun; F Farabegoli; A Pession; F Novello
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

8.  Osteopontin is histochemically detected by the AgNOR acid-silver staining.

Authors:  C Gaudin-Audrain; Y Gallois; F Pascaretti-Grizon; L Hubert; P Massin; M-F Baslé; D Chappard
Journal:  Histol Histopathol       Date:  2008-04       Impact factor: 2.303

Review 9.  Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry.

Authors:  M Derenzini; A L Olins; D E Olins
Journal:  Eur J Histochem       Date:  2014-01-24       Impact factor: 3.188
  9 in total

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