Literature DB >> 6639038

Simple and rapid method for disruption of bacteria for protein studies.

S Bhaduri, P H Demchick.   

Abstract

A simple and rapid method was developed for the extraction of proteins from both pathogenic and nonpathogenic bacteria. The method involves the treatment of cells with acetone followed by sodium dodecyl sulfate extraction of cellular proteins. Polyacrylamide gel electrophoresis revealed that the protein composition of extracts made by this method was comparable to that of extracts made by established methods, namely, sonication and agitation with beads. This technique has been successfully applied to the extraction of proteins from a wide variety of bacteria, including pathogens.

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Year:  1983        PMID： 6639038      PMCID： PMC239492          DOI： 10.1128/aem.46.4.941-943.1983

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  EFFECT OF ARGININE ON GROWTH AND LYSIS OF CLOSTRIDIUM BOTULINUM.

Authors:  L E BOWERS; O B WILLIAMS
Journal:  J Bacteriol       Date:  1963-05       Impact factor: 3.490

2.  Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.

Authors:  G F Ames
Journal:  J Biol Chem       Date:  1974-01-25       Impact factor: 5.157

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Achromopeptidase for lysis of anaerobic gram-positive cocci.

Authors:  T Ezaki; S Suzuki
Journal:  J Clin Microbiol       Date:  1982-11       Impact factor: 5.948

5.  Charge separation of proteins complexed with sodium dodecyl sulfate by acid gel electrophoresis in the presence of cetyltrimethylammonium bromide.

Authors:  J J Basch; H M Farrell
Journal:  Biochim Biophys Acta       Date:  1979-03-27

6.  Procedure for the preparation of milligram quantities of adenovirus messenger ribonucleic acid.

Authors:  S Bhaduri; H J Raskas; M Green
Journal:  J Virol       Date:  1972-12       Impact factor: 5.103

7.  Uptake and metabolism of sucrose by Streptococcus lactis.

Authors:  J Thompson; B M Chassy
Journal:  J Bacteriol       Date:  1981-08       Impact factor: 3.490
  7 in total
  18 in total

1.  Acquired resistance of Nocardia brasiliensis to clavulanic acid related to a change in beta-lactamase following therapy with amoxicillin-clavulanic acid.

Authors:  V A Steingrube; R J Wallace; B A Brown; Y Pang; B Zeluff; L C Steele; Y Zhang
Journal:  Antimicrob Agents Chemother       Date:  1991-03       Impact factor: 5.191

2.  Growth, Fe3+ reductase activity, and siderophore production by Paenibacillus polymyxa SQR-21 under differential iron conditions.

Authors:  Waseem Raza; Qirong Shen
Journal:  Curr Microbiol       Date:  2010-04-01       Impact factor: 2.188

3.  DNA fingerprinting by infrequent-restriction-site amplification.

Authors:  G H Mazurek; V Reddy; B J Marston; W H Haas; J T Crawford
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

4.  Regulation of exoprotein gene expression in Staphylococcus aureus by agar.

Authors:  P Recsei; B Kreiswirth; M O'Reilly; P Schlievert; A Gruss; R P Novick
Journal:  Mol Gen Genet       Date:  1986-01

5.  Phenotypic and molecular characterization of native Azospirillum strains from rice fields to improve crop productivity.

Authors:  Ranjan K Sahoo; Mohammad W Ansari; Madhusmita Pradhan; Tushar K Dangar; Santanu Mohanty; Narendra Tuteja
Journal:  Protoplasma       Date:  2014-01-12       Impact factor: 3.356

6.  A site-specific recombination function in Staphylococcus aureus plasmids.

Authors:  M L Gennaro; J Kornblum; R P Novick
Journal:  J Bacteriol       Date:  1987-06       Impact factor: 3.490

7.  A simple and generally applicable procedure for releasing DNA from bacterial cells.

Authors:  L S Heath; G L Sloan; H E Heath
Journal:  Appl Environ Microbiol       Date:  1986-05       Impact factor: 4.792

8.  Phenotypic and molecular characterisation of efficient nitrogen-fixing Azotobacter strains from rice fields for crop improvement.

Authors:  Ranjan K Sahoo; Mohammad W Ansari; Tushar K Dangar; Santanu Mohanty; Narendra Tuteja
Journal:  Protoplasma       Date:  2013-09-05       Impact factor: 3.356

9.  BRO beta-lactamases of Branhamella catarrhalis and Moraxella subgenus Moraxella, including evidence for chromosomal beta-lactamase transfer by conjugation in B. catarrhalis, M. nonliquefaciens, and M. lacunata.

Authors:  R J Wallace; V A Steingrube; D R Nash; D G Hollis; C Flanagan; B A Brown; A Labidi; R E Weaver
Journal:  Antimicrob Agents Chemother       Date:  1989-11       Impact factor: 5.191

10.  Precipitation of cadmium by Clostridium thermoaceticum.

Authors:  D P Cunningham; L L Lundie
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792
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