| Literature DB >> 6638492 |
M Badiani, M Felici, M Luna, F Artemi.
Abstract
Spectrophotometric determination of laccase activity may be affected by the formation of quinoid chromophores arising from nonenzymatic oxidations interfering with enzymatic reactions. Km values for guaiacol obtained by spectrophotometric and HPLC methods confirm the above hypothesis. HPLC results are particularly useful for the assay of laccase activity on natural phenolic extracts.Entities:
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Year: 1983 PMID： 6638492 DOI： 10.1016/0003-2697(83)90082-9
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365