Literature DB >> 6638480

Methylation analysis of complex carbohydrates in small amounts: capillary gas chromatography-mass fragmentography of methylalditol acetates obtained from N-glycosidically linked glycoprotein oligosaccharides.

R Geyer, H Geyer, S Kühnhardt, W Mink, S Stirm.   

Abstract

A version of the methylation analysis of complex carbohydrates by gas chromatography-mass spectrometry of the methylalditol acetates (H. Björndal, C. G. Hellerquist, B. Lindberg, and S. Svensson (1970) Angew. Chem. 82, 643-674) is described. With this version 100- to 500-pmol samples of N-glycosidically linked glycoprotein oligosaccharides may be analyzed. The method is based on the use of capillary columns which allow the separation of all partially methylated alditol acetates potentially obtained from this group of oligosaccharides and on their selective and sensitive detection by mass fragmentography after chemical ionization with ammonia.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6638480     DOI： 10.1016/0003-2697(83)90243-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

Review 1.  Carbohydrate analysis of glycoproteins. A review.

Authors:  K B Lee; D Loganathan; Z M Merchant; R J Linhardt
Journal:  Appl Biochem Biotechnol       Date:  1990-01       Impact factor: 2.926

2.  The alpha-D-mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for beta-glucosidase activation.

Authors:  J Rath; R Messner; P Kosma; F Altmann; L März; C P Kubicek
Journal:  Arch Microbiol       Date:  1995-12       Impact factor: 2.552

3.  Glycosylation of the thrombin-like serine protease ancrod from Agkistrodon rhodostoma venom. Oligosaccharide substitution pattern at each N-glycosylation site.

Authors:  G Pfeiffer; D Linder; K H Strube; R Geyer
Journal:  Glycoconj J       Date:  1993-06       Impact factor: 2.916

4.  Comparison of the carbohydrate moieties of recombinant soluble Fc epsilon receptor (sFc epsilon RII/sCD23) expressed in Saccharomyces cerevisiae and Chinese hamster ovary cells. Different O-glycosylation sites are used by yeast and mammalian cells.

Authors:  I Kalsner; F J Schneider; R Geyer; H Ahorn; I Maurer-Fogy
Journal:  Glycoconj J       Date:  1992-08       Impact factor: 2.916

5.  Alpha 1-6(alpha 1-3)-difucosylation of the asparagine-bound N-acetylglucosamine in honeybee venom phospholipase A2.

Authors:  E Staudacher; F Altmann; L März; K Hård; J P Kamerling; J F Vliegenthart
Journal:  Glycoconj J       Date:  1992-04       Impact factor: 2.916

6.  Carbohydrate structure of human pancreatic elastase 1.

Authors:  P Wendorf; D Linder; A Sziegoleit; R Geyer
Journal:  Biochem J       Date:  1991-09-01       Impact factor: 3.857

7.  Structural studies of a mannitol teichoic acid from the cell wall of bacterium N.C.T.C. 9742.

Authors:  W J Anderton; S G Wilkinson
Journal:  Biochem J       Date:  1985-03-01       Impact factor: 3.857

8.  Detailed structural characterization of succinoglycan, the major exopolysaccharide of Rhizobium meliloti Rm1021.

Authors:  B B Reinhold; S Y Chan; T L Reuber; A Marra; G C Walker; V N Reinhold
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

9.  Carbohydrates of influenza virus. Structural elucidation of the individual glycans of the FPV hemagglutinin by two-dimensional 1H n.m.r. and methylation analysis.

Authors:  W Keil; R Geyer; J Dabrowski; U Dabrowski; H Niemann; S Stirm; H D Klenk
Journal:  EMBO J       Date:  1985-10       Impact factor: 11.598

10.  The carbohydrates of mouse hepatitis virus (MHV) A59: structures of the O-glycosidically linked oligosaccharides of glycoprotein E1.

Authors:  H Niemann; R Geyer; H D Klenk; D Linder; S Stirm; M Wirth
Journal:  EMBO J       Date:  1984-03       Impact factor: 11.598
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.