Literature DB >> 6634410

A stereospecifically 18O-labelled deoxydinucleoside phosphate block for incorporation into an oligonucleotide.

B V Potter, F Eckstein, B Uznański.   

Abstract

Fully protected diastereoisomers of deoxyguanylyl (3' leads to 5') deoxyadenosine stereospecifically labelled on phosphorus with oxygen-18 have been synthesized by oxidation of phosphite triester intermediates in the presence of 18O-labelled water. The diastereoisomers have been chromatographically separated and their absolute configuration at phosphorus determined. (Rp)-[18O]deoxyguanylyl (3' leads to 5')deoxyadenosine has been prepared by complete deprotection of the parent diastereoisomer of the Sp configuration. Methylation of the former compound permits assignment of the absolute configurations of the methyl esters of N1-methyldeoxyguanylyl (3' leads to 5') N1-methyldeoxyadenosine.

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Year:  1983        PMID： 6634410      PMCID： PMC326440          DOI： 10.1093/nar/11.20.7087

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate.

Authors:  P H Greene; M S Poonian; A L Nussbaum; L Tobias; D E Garfin; H W Boyer; H M Goodman
Journal:  J Mol Biol       Date:  1975-12-05       Impact factor: 5.469

2.  Synthesis of thymidine oligonucleotides by phosphite triester intermediates.

Authors:  R L Letsinger; W B Lunsford
Journal:  J Am Chem Soc       Date:  1976-06-09       Impact factor: 15.419

3.  Synthesis of oligoribonucleotides based on the facile cleavage of methyl phosphotriester intermediates.

Authors:  G W Daub; E E van Tamelen
Journal:  J Am Chem Soc       Date:  1977-05-11       Impact factor: 15.419

Review 4.  Enzyme-catalyzed phosphoryl transfer reactions.

Authors:  J R Knowles
Journal:  Annu Rev Biochem       Date:  1980       Impact factor: 23.643

5.  Stereochemistry of enzymic phosphoryl and nucleotidyl transfer.

Authors:  F Eckstein; P J Romaniuk; B A Connolly
Journal:  Methods Enzymol       Date:  1982       Impact factor: 1.600

6.  The interaction of the EcoRI restriction endonuclease with its substrate. A physico-chemical study employing natural and synthetic oligonucleotides and polynucleotides.

Authors:  M Goppelt; A Pingoud; G Maass; H Mayer; H Köster; R Frank
Journal:  Eur J Biochem       Date:  1980-02

7.  Interaction of the HpaI endonuclease with synthetic oligonucleotides.

Authors:  P Dwyer-Hallquist; F J Kézdy; K L Agarwal
Journal:  Biochemistry       Date:  1982-09-14       Impact factor: 3.162

8.  Synthesis and configurational analysis of a dinucleoside phosphate isotopically chiral at phosphorus. Stereochemical course of Penicillium citrum nuclease P1 reaction.

Authors:  B V Potter; B A Connolly; F Eckstein
Journal:  Biochemistry       Date:  1983-03-15       Impact factor: 3.162
  8 in total
  7 in total

1.  The estimation of distances between specific backbone-labeled sites in DNA using fluorescence resonance energy transfer.

Authors:  H Ozaki; L W McLaughlin
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

2.  Alkyl phosphotriester modified oligodeoxyribonucleotides. V. Synthesis and absolute configuration of Rp and Sp diastereomers of an ethyl phosphotriester (Et) modified EcoRI recognition sequence, d[GGAA(Et)TTCC]. A synthetic approach to regio- and stereospecific ethylation-interference studies.

Authors:  K A Gallo; K L Shao; L R Phillips; J B Regan; M Koziolkiewicz; B Uznanski; W J Stec; G Zon
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

3.  Mung bean (Phaseolus aureus) nuclease. A mechanistic investigation of the DNA-cleavage reaction using a dinucleoside phosphorothioate.

Authors:  M R Hamblin; J H Cummins; B V Potter
Journal:  Biochem J       Date:  1987-02-01       Impact factor: 3.857

4.  Alkyl phosphotriester modified oligodeoxyribonucleotides. VI. NMR and UV spectroscopic studies of ethyl phosphotriester (Et) modified Rp-Rp and Sp-Sp duplexes, (d[GGAA(Et)TTCC])2.

Authors:  M F Summers; C Powell; W Egan; R A Byrd; W D Wilson; G Zon
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

5.  Inhibition of deoxyribonucleases by phosphorothioate groups in oligodeoxyribonucleotides.

Authors:  S Spitzer; F Eckstein
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

6.  Synthesis of ¹⁸O-labeled RNA for application to kinetic studies and imaging.

Authors:  Tomohiro Hamasaki; Takahiro Matsumoto; Naoya Sakamoto; Akiko Shimahara; Shiori Kato; Ayumi Yoshitake; Ayumi Utsunomiya; Hisayoshi Yurimoto; Esteban C Gabazza; Tadaaki Ohgi
Journal:  Nucleic Acids Res       Date:  2013-04-30       Impact factor: 16.971

7.  Pinpointing RNA-Protein Cross-Links with Site-Specific Stable Isotope-Labeled Oligonucleotides.

Authors:  Victor S Lelyveld; Anders Björkbom; Elizabeth M Ransey; Piotr Sliz; Jack W Szostak
Journal:  J Am Chem Soc       Date:  2015-12-03       Impact factor: 15.419
  7 in total

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