Control of the changes in rat-liver carbamoyl-phosphate synthase (ammonia) protein levels during ontogenesis: evidence for a perinatal change in immunoreactivity of the enzyme.

To analyze the changes in rat-liver carbamoyl-phosphate synthase (Cpase) protein levels during ontogenesis, these levels were determined by means of two independent methods, i.e. radioimmunoassay and densitometric assay. During normal development the changes in catalytic activity of Cpase are accompanied by equivalent changes in the quantities of enzyme protein. We have obtained evidence for the existence of a perinatal Cpase which is immunochemically different from adult Cpase as immunoreactivity of Cpase decreases in the perinatal period and remains constant thereafter.