Literature DB >> 6631633

High efficiency plating method for Leishmania promastigotes in semidefined or completely-defined medium.

D M Iovannisci, B Ullman.   

Abstract

A simple technique, developed for the isolation of clones derived from single, promastigote cells of Leishmania donovani and Leishmania tropica, involved the use of semisolid agar. Both species of Leishmania promastigotes formed discrete colonies at high efficiency either in semidefined medium containing 10% fetal calf serum or in completely-defined medium lacking serum. Visible colonies appeared between 8 and 14 days in growth medium containing 10% fetal calf serum. Replacement of the fetal calf serum with bovine serum albumin and Tween-80 increased the time of colony formation by 50% but did not affect the cloning efficiency. Viability of colonies transferred from semisolid agar to liquid suspension culture was 100%.

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Year:  1983        PMID： 6631633

Source DB:  PubMed          Journal:  J Parasitol        ISSN： 0022-3395            Impact factor:   1.276


  40 in total

1.  Adaptive responses to purine starvation in Leishmania donovani.

Authors:  Nicola S Carter; Phillip A Yates; Sarah K Gessford; Sean R Galagan; Scott M Landfear; Buddy Ullman
Journal:  Mol Microbiol       Date:  2010-10       Impact factor: 3.501

2.  Point mutations in a nucleoside transporter gene from Leishmania donovani confer drug resistance and alter substrate selectivity.

Authors:  G Vasudevan; B Ullman; S M Landfear
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-15       Impact factor: 11.205

3.  High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates.

Authors:  V B Carruthers; G A Cross
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

4.  Leishmania donovani polyamine biosynthetic enzyme overproducers as tools to investigate the mode of action of cytotoxic polyamine analogs.

Authors:  Sigrid C Roberts; Yuqui Jiang; Judith Gasteier; Benjamin Frydman; Laurence J Marton; Olle Heby; Buddy Ullman
Journal:  Antimicrob Agents Chemother       Date:  2006-11-20       Impact factor: 5.191

5.  LRV1 viral particles in Leishmania guyanensis contain double-stranded or single-stranded RNA.

Authors:  R Weeks; R F Aline; P J Myler; K Stuart
Journal:  J Virol       Date:  1992-03       Impact factor: 5.103

6.  High-affinity binding of silybin derivatives to the nucleotide-binding domain of a Leishmania tropica P-glycoprotein-like transporter and chemosensitization of a multidrug-resistant parasite to daunomycin.

Authors:  J M Pérez-Victoria; F J Pérez-Victoria; G Conseil; M Maitrejean; G Comte; D Barron; A Di Pietro; S Castanys; F Gamarro
Journal:  Antimicrob Agents Chemother       Date:  2001-02       Impact factor: 5.191

7.  Genetic dissection of pyrimidine biosynthesis and salvage in Leishmania donovani.

Authors:  Zachary N Wilson; Caslin A Gilroy; Jan M Boitz; Buddy Ullman; Phillip A Yates
Journal:  J Biol Chem       Date:  2012-02-24       Impact factor: 5.157

8.  Characterization of sinefungin-resistant Leishmania donovani promastigotes.

Authors:  M A Phelouzat; F Lawrence; M Robert-Gero
Journal:  Parasitol Res       Date:  1993       Impact factor: 2.289

9.  Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant.

Authors:  G Vasudevan; N S Carter; M E Drew; S M Beverley; M A Sanchez; A Seyfang; B Ullman; S M Landfear
Journal:  Proc Natl Acad Sci U S A       Date:  1998-08-18       Impact factor: 11.205

10.  Multidrug resistance in Leishmania donovani is conferred by amplification of a gene homologous to the mammalian mdr1 gene.

Authors:  D M Henderson; C D Sifri; M Rodgers; D F Wirth; N Hendrickson; B Ullman
Journal:  Mol Cell Biol       Date:  1992-06       Impact factor: 4.272
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