Literature DB >> 6630868

Removal of fatty acids from serum albumin by Lipidex 1000 chromatography.

J F Glatz, J H Veerkamp.   

Abstract

Fatty acids can be effectively removed from serum albumin preparations by a single passage through a column of Lipidex 1000 at 37 degrees C. The procedure is easier and milder and shows a better (nearly quantitative) recovery of protein than charcoal treatment. The ability for fatty acid binding by the protein is not affected by either procedure.

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Year:  1983        PMID: 6630868     DOI: 10.1016/0165-022x(83)90021-0

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  13 in total

1.  Osmolyte perturbation reveals conformational equilibria in spin-labeled proteins.

Authors:  Carlos J López; Mark R Fleissner; Zhefeng Guo; Ana K Kusnetzow; Wayne L Hubbell
Journal:  Protein Sci       Date:  2009-08       Impact factor: 6.725

2.  Structural insights into human serum albumin-mediated prostaglandin catalysis.

Authors:  Jinsheng Yang; Charles E Petersen; Chung-Eun Ha; Nadhipuram V Bhagavan
Journal:  Protein Sci       Date:  2002-03       Impact factor: 6.725

3.  Five recombinant fragments of human serum albumin-tools for the characterization of the warfarin binding site.

Authors:  M Dockal; M Chang; D C Carter; F Rüker
Journal:  Protein Sci       Date:  2000-08       Impact factor: 6.725

4.  Solution structure and backbone dynamics of human epidermal-type fatty acid-binding protein (E-FABP).

Authors:  Luis H Gutiérrez-González; Christian Ludwig; Carsten Hohoff; Martin Rademacher; Thorsten Hanhoff; Heinz Rüterjans; Friedrich Spener; Christian Lücke
Journal:  Biochem J       Date:  2002-06-15       Impact factor: 3.857

5.  Millisecond timescale dynamics of human liver fatty acid binding protein: testing of its relevance to the ligand entry process.

Authors:  Dong Long; Daiwen Yang
Journal:  Biophys J       Date:  2010-06-16       Impact factor: 4.033

6.  Assay of the binding of fatty acids by proteins: evaluation of the Lipidex 1000 procedure.

Authors:  M M Vork; J F Glatz; D A Surtel; G J van der Vusse
Journal:  Mol Cell Biochem       Date:  1990 Oct 15-Nov 8       Impact factor: 3.396

7.  Spin-system heterogeneities indicate a selected-fit mechanism in fatty acid binding to heart-type fatty acid-binding protein (H-FABP).

Authors:  C Lücke; M Rademacher; A W Zimmerman; H T van Moerkerk; J H Veerkamp; H Rüterjans
Journal:  Biochem J       Date:  2001-03-01       Impact factor: 3.857

8.  Buffer interference with protein dynamics: a case study on human liver fatty acid binding protein.

Authors:  Dong Long; Daiwen Yang
Journal:  Biophys J       Date:  2009-02-18       Impact factor: 4.033

9.  The NMR structure of a stable and compact all-beta-sheet variant of intestinal fatty acid-binding protein.

Authors:  Benhur Ogbay; Gregory T Dekoster; David P Cistola
Journal:  Protein Sci       Date:  2004-05       Impact factor: 6.725

10.  Cell surface hydrophobicity of Bifidobacterium bifidum subsp. pennsylvanicum.

Authors:  H J Op den Camp; A Oosterhof; J H Veerkamp
Journal:  Antonie Van Leeuwenhoek       Date:  1985       Impact factor: 2.271

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