High-performance liquid chromatography of amino acids, peptides and proteins. XLVIII. Retention behaviour of tryptic peptides of human growth hormone isolated by reversed-phase high-performance liquid chromatography: a comparative study using different chromatographic conditions and predicted elution behaviour based on retention coefficients.

The retention behaviour of the tryptic peptides of human growth hormone under a variety of reversed-phase chromatographic conditions has been investigated. By using water-acetonitrile gradients containing phosphate, bicarbonate, trifluoroacetate, or heptanesulphonate buffers, the influence of these ionic modifiers on peptide selectivity with several different alkylsilicas has been examined. Comparisons of observed and predicted retention behaviours for the various tryptic peptides in the different chromatographic systems are described. Application of these systems to the isolation of deamidated forms of human growth hormone is presented.