Literature DB >> 6630360

Isolation of thymosin alpha 1 from thymosin fraction 5 of different species by high-performance liquid chromatography.

T L Low, J E McClure, P H Naylor, B L Spangelo, A L Goldstein.   

Abstract

High-performance liquid chromatography (muBondapak C18 column with 0.05% trifluoroacetic acid in acetonitrile as solvent system) was used to isolate thymosin alpha 1 (alpha 1) from thymosin fraction 5 (f5) of various species (calf, pig, sheep and mouse). Each of the f5 preparations gave a protein peak similar in retention time to bovine thymosin alpha 1. This peak coincided with the immunoreactive peak determined by a radioimmunoassay for alpha 1. Chromatographic analysis of fresh thymus tissue extracts using a high-performance liquid chromatographic similar system did not reveal a detectable protein peak or immunoreactive peak at the alpha 1 position. Our results suggest that alpha 1 may be synthesized in a precursor form in animal tissues.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6630360     DOI： 10.1016/s0021-9673(01)90924-0

Source DB:  PubMed          Journal:  J Chromatogr


  6 in total

1.  Quantitative analysis of thymosin alpha1 in human serum by LC-MS/MS.

Authors:  C W Tuthill; A Rudolph; Y Li; B Tan; T J Fitzgerald; S R Beck; Y X Li
Journal:  AAPS PharmSciTech       Date:  2000-05-14       Impact factor: 3.246

2.  Molecular cloning of cDNA for human prothymosin alpha.

Authors:  G J Goodall; F Dominguez; B L Horecker
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

3.  Prothymosin alpha in human blood.

Authors:  C Panneerselvam; A A Haritos; J Caldarella; B L Horecker
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 11.205

4.  Distribution of prothymosin alpha in rat tissues.

Authors:  A A Haritos; O Tsolas; B L Horecker
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

5.  Production of Nα-acetylated thymosin α1 in Escherichia coli.

Authors:  Yuantao Ren; Xueqin Yao; Hongmei Dai; Shulong Li; Hongqing Fang; Huipeng Chen; Changlin Zhou
Journal:  Microb Cell Fact       Date:  2011-04-22       Impact factor: 5.328

6.  Expression, purification and characterization of a novel soluble human thymosin alpha1 concatemer exhibited a stronger stimulation on mice lymphocytes proliferation and higher anti-tumor activity.

Authors:  Weina Li; Liqiang Song; Shouzhen Wu; Xiaochang Xue; Lu Zhang; Liqing He; Wei Han; Qing Wang; Rui Ling; Wei Zhang; Zhen Yan; Yingqi Zhang
Journal:  Int J Biol Sci       Date:  2011-05-19       Impact factor: 6.580
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.