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Literature DB >> 6628160

Failure of inhibitor assay to determine isoamylase distribution.

Abstract

Isoamylase distribution in the urine of normal individuals and patients admitted to the hospital with hyperamylasemia was determined by a wheat inhibitor mmethod and compared to results obtained by cellulose-acetate electrophoresis. We report two cases where the inhibitor ethod failed to give accurate results in urine, as well as serum, when compared to both electrophoresis and column chromatography. The discrepant results were due to the unexpected inhibition of P isoamylase.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1983 PMID： 6628160 DOI： 10.1007/bf01311727

Source DB: PubMed Journal: Dig Dis Sci ISSN： 0163-2116 Impact factor: 3.199

5 in total

1. Purification and properties of an alpha-amylase inhibitor from wheat.

Authors: M D O'Donnell; K F McGeeney
Journal: Biochim Biophys Acta Date: 1976-01-23

2. Simplified chromatographic method for isoamylase analysis.

Authors: L Fridhandler; J E Berk
Journal: Clin Chim Acta Date: 1980-02-14 Impact factor: 3.786

3. Electrophoretic amylase fractionation as an aid in diagnosis of pancreatic disease.

Authors: M E Legaz; M A Kenny
Journal: Clin Chem Date: 1976-01 Impact factor: 8.327

4. Evaluation of an inhibitor assay to determine serum isoamylase distribution.

Authors: C Ellis; D F Koehler; J H Eckfeldt; M D Levitt
Journal: Dig Dis Sci Date: 1982-10 Impact factor: 3.199

5. Inhibitor test for amylase isoenzymes: comparison with a simplified chromatographic method.

Authors: J E Berk; D Simon; L Fridhandler
Journal: Am J Gastroenterol Date: 1981-02 Impact factor: 10.864

5 in total

1. Isoamylase determination by isoelectric focusing in pancreatic disorders. A potential clinical aid.

Authors: G Chiarioni; B Vaona; L Benini; G Dimitri; C Scattolini; L A Scuro; I Vantini
Journal: Int J Pancreatol Date: 1991-01

Review 2. Laboratory tests in the diagnosis of the chronic pancreatic diseases. Part 4. Tests involving the measurement of pancreatic enzymes in body fluid.

Authors: E J Boyd; H Rinderknecht; K G Wormsley
Journal: Int J Pancreatol Date: 1988 Jan-Feb