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Literature DB >> 6626176

Characterization of sea-urchin fibronectin.

Abstract

Sea-urchin fibronectin from the ovary of the sea urchin Pseudocentrotus depressus bound to gelatin, fibrin and fibrinogen. After mild digestion of the protein labelled with 125I, a 195 000 Da domain was observed. Sea-urchin fibronectin was aggregated by spermine (1 mM) at neutral pH. When the concentration of spermine was decreased or increased, the aggregation was diminished. The addition of 1 M-NaCl or 4 M-urea inhibited the spermine-induced aggregation. Sea-urchin fibronectin mediated the spreading of baby-hamster kidney cells on the plastic surface.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1983 PMID： 6626176 PMCID： PMC1152382 DOI： 10.1042/bj2150205

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

Authors: R A Laskey; A D Mills
Journal: Eur J Biochem Date: 1975-08-15

2. Fibroblast surface antigen (SF): the external glycoprotein lost in proteolytic stimulation and maligant transfromation.

Authors: J Keski-Oja; A Vaheri; E Ruoslahti
Journal: Int J Cancer Date: 1976-02-15 Impact factor: 7.396

Characterization of sea-urchin fibronectin.

1. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

2. Fibroblast surface antigen (SF): the external glycoprotein lost in proteolytic stimulation and maligant transfromation.

3. Binding of soluble form of fibroblast surface protein, fibronectin, to collagen.

4. Localization of the binding site for cell attachment in the alpha1(I) chain of collagen.

5. Cell adhesion and spreading factor. Partial purification and properties.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Cross-linking of cold-insoluble globulin by fibrin-stabilizing factor.

8. Cross-linking of fibronectin to sulfated proteoglycans at the cell surface.

9. Domain structure of fibronectin and its relation to function. Disulfides and sulfhydryl groups.

10. Dissociation of fibronectin from gelatin-agarose by amino compounds.

1. Biochemical and immunological relationships among fibronectin-like proteins from different sea urchin species.

2. Role of fibronectin in primary mesenchyme cell migration in the sea urchin.

3. Isolation and purification of a cell adhesion factor from crayfish blood cells.