Cytogenetic effects of an alkyl-lysophospholipid derivative in human peripheral lymphocytes exposed in vitro and in vivo.

Chromosome analyses were carried out in human lymphocytes exposed to an antitumor alkyl-lysophospholipid (ALP) in vitro and in vivo. There was no evidence for a significant clastogenic effect or incidence of an increased sister-chromatid exchange (SCE) after short-term and long-term treatment of unstimulated or stimulated lymphocytes with concentrations of 0.1-50 micrograms ALP per ml. Cell proliferation was delayed after G0 exposure to 50 micrograms/ml. Although no ALP monotherapy was present for 3 cancer patients examined before and during ALP treatment with accumulated doses of 15-516 mg/kg, the cytogenetic data were in accord with the negative experimental results.