| Literature DB >> 6619119 |
Y Hirabayashi, T Suzuki, Y Suzuki, T Taki, M Matsumoto, H Higashi, S Kato.
Abstract
A new method for purification of anti-glycosphingolipid antibodies had been developed. N-Glycolylneuraminyl(alpha 2-3)lactosylceramide [hematoside (NeuGc)] could be hydrophobically bound on octyl-Sepharose 4B in the presence of 0.1 M KCl. The Sepharose gel coated with hematoside (NeuGc) was used as immunoadsorbent for affinity column chromatography to purify avian anti-hematoside (NeuGc) antibody. The procedure is very simple, reproducible and applicable to purification of almost all anti-glycosphingolipid antibodies. The glycosphingolipid used for the affinity chromatography could be recovered without any destruction by successive extraction of the gel with methanol and methanol/chloroform (1:2, v/v).Entities:
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Year: 1983 PMID: 6619119 DOI: 10.1093/oxfordjournals.jbchem.a134350
Source DB: PubMed Journal: J Biochem ISSN: 0021-924X Impact factor: 3.387