Literature DB >> 6617765

Analysis of proportion regulation in slugs of Dictyostelium discoideum using a monoclonal antibody and a FACS-IV.

M Krefft, L Voet, H Mairhofer, K L Williams.   

Abstract

A quantitative assay for estimating the proportion of prespore cells in D. discoideum slugs was established by labelling disaggregated slug cells with a prespore specific monoclonal antibody and analysing the cell population with a FACS-IV. The method is validated using a wild-type strain and its stalky mutant. "Wild-type" strains have different proportions of prespore cells and it is demonstrated that slugs of some strains have an increased percentage of prespore cells when migrated in the dark compared to the light and in the presence of EGTA. The technique is rapid and will make possible genetic analysis of proportion regulation in D. discoideum.

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Year:  1983        PMID： 6617765     DOI： 10.1016/0014-4827(83)90291-4

Source DB:  PubMed          Journal:  Exp Cell Res        ISSN： 0014-4827            Impact factor:   3.905


  7 in total

1.  Chemotactic sorting to cAMP in the multicellular stages of Dictyostelium development.

Authors:  D Traynor; R H Kessin; J G Williams
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-01       Impact factor: 11.205

2.  Size polymorphisms due to changes in the number of O-glycosylated tandem repeats in the Dictyostelium discoideum glycoprotein PsA.

Authors:  A A Gooley; R Marshchalek; K L Williams
Journal:  Genetics       Date:  1992-04       Impact factor: 4.562

3.  A polymorphic, prespore-specific cell surface glycoprotein is present in the extracellular matrix of Dictyostelium discoideum.

Authors:  W N Grant; D L Welker; K L Williams
Journal:  Mol Cell Biol       Date:  1985-10       Impact factor: 4.272

4.  Copine A plays a role in the differentiation of stalk cells and the initiation of culmination in Dictyostelium development.

Authors:  Tasha S Smith; Jaimie M Pineda; Alex C Donaghy; Cynthia K Damer
Journal:  BMC Dev Biol       Date:  2010-06-02       Impact factor: 1.978

5.  Structural characterization of Dictyostelium discoideum prespore-specific gene D19 and of its product, cell surface glycoprotein PsA.

Authors:  A E Early; J G Williams; H E Meyer; S B Por; E Smith; K L Williams; A A Gooley
Journal:  Mol Cell Biol       Date:  1988-08       Impact factor: 4.272

6.  Evidence that positional information is used to establish the prestalk-prespore pattern in Dictyostelium discoideum aggregates.

Authors:  M Krefft; L Voet; J H Gregg; H Mairhofer; K L Williams
Journal:  EMBO J       Date:  1984-01       Impact factor: 11.598

7.  Activation of the prespore and spore cell pathway of Dictyostelium differentiation by cAMP-dependent protein kinase and evidence for its upstream regulation by ammonia.

Authors:  N A Hopper; A J Harwood; S Bouzid; M Véron; J G Williams
Journal:  EMBO J       Date:  1993-06       Impact factor: 11.598
  7 in total

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