The nucleotide sequence of methionine elongator tRNA from wheat germ.

Wheat germ methionine elongator tRNA (tRNAmMet) was purified by three column chromatogaphies followed by electrophoresis on polyacrylamide gel. Its sequence is pG-G-G-G-U-G-G-U-m1G-m2G-C-G-C-A-G-D-D-G-G-C-acp3U-A-G-C-G-C-m22G-psi-A-G-G- psi-C-U-Cm-A-U-mt6A-A-psi-C-C-U-G-A-G-m7G-D-m5C-G-A-G-A-T-psi-C-G-m1A-G2-C-C-U- C-U-C-U-C-A-C-C-C-C-A-C-C-A. Two hypermodified nucleosides, methylthreoninocarbonyladenosine (mt6A) and 3(3-amino-3-carboxypropyl)uridine (acp3U), are present in this tRNA.