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Literature DB >> 6615447

Development of a radioimmunoassay for rat liver metallothionein-I and its application to the analysis of rat plasma and kidneys.

Abstract

A sensitive radioimmunoassay for rat liver metallothionein-I has been developed using avid and high-titre antibodies obtained from sheep that were immunized with a conjugate of metallothionein and rabbit immunoglobulin G. The assay was specific for metallothionein-I, and did not depend on the particular metal bound to the protein. There was no significant cross-reaction with rat liver metallothionein-II. The use of the assay to measure metallothionein concentrations in rat plasma and kidneys is described.

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Year: 1983 PMID： 6615447 PMCID： PMC1152148 DOI： 10.1042/bj2130459

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

21 in total

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Authors: J S Garvey; R J Vander Mallie; C C Chang
Journal: Methods Enzymol Date: 1982 Impact factor: 1.600

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Authors: M Vasák; A Galdes; H A Hill; J H Kägi; I Bremner; B W Young
Journal: Biochemistry Date: 1980-02-05 Impact factor: 3.162

6. Ammonium sulfate and polyethylene glycol as reagents to separate antigen from antigen-antibody complexes.

Authors: T Chard
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

7. Transcriptional regulation of the mouse metallothionein-I gene by heavy metals.

Authors: D M Durnam; R D Palmiter
Journal: J Biol Chem Date: 1981-06-10 Impact factor: 5.157

8. The effects of age, sex, and zinc status on the accumulation of (copper, zinc)- metallothionein in rat kidneys.

Authors: I Bremner; R B Williams; B W Young
Journal: J Inorg Biochem Date: 1981-04 Impact factor: 4.155

9. Detection of circulating metallothionein in rats injected with zinc or cadmium.

Authors: J S Garvey; C C Chang
Journal: Science Date: 1981-11-13 Impact factor: 47.728

10. Metallothionein in plasma and urine of cadmium-exposed rats determined by a single-antibody radioimmunoassay.

Authors: C Tohyama; Z A Shaikh
Journal: Fundam Appl Toxicol Date: 1981 Jan-Feb

18 in total

1. A gel filtration high-performance liquid chromatographic method for determination of hepatic and renal metallothionein of rat and in comparison with the cadmium-saturation method.

Authors: N Jin; M Kimura; K Yokoi; Y Itokawa
Journal: Biol Trace Elem Res Date: 1993-02 Impact factor: 3.738

2. Radioimmunoassay of metallothionein in rabbit, rat, mouse, Chinese hamster, and human cells.

Authors: M E Leibrandt; J Koropatnick; J F Harris; M G Cherian
Journal: Biol Trace Elem Res Date: 1991-09 Impact factor: 3.738

3. Evidence for differences in the post-transcriptional regulation of rat metallothionein isoforms.

Authors: M H Vasconcelos; S C Tam; J H Beattie; J E Hesketh
Journal: Biochem J Date: 1996-04-15 Impact factor: 3.857

4. Histological demonstration of immunoreactive zinc metallothionein in liver and ileum of rat and man.

Authors: J P Clarkson; M E Elmes; B Jasani; M Webb
Journal: Histochem J Date: 1985-03

5. Histological demonstration of immunoreactive metallothionein in sections of human and rat tissue.

Authors: M E Elmes; J P Clarkson; B Jasani
Journal: Environ Geochem Health Date: 1985-12 Impact factor: 4.609

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Authors: D Holt; S Sparrow; M Webb
Journal: Arch Toxicol Date: 1985-08 Impact factor: 5.153

7. Copper binding components of blood plasma and organs, and their responses to influx of large doses of (65)Cu, in the mouse.

Authors: Anthony Cabrera; Erin Alonzo; Eric Sauble; Yu Ling Chu; Dionne Nguyen; Maria C Linder; Dee S Sato; Andrew Z Mason
Journal: Biometals Date: 2008-03-21 Impact factor: 2.949