Literature DB >> 66149

Immunoglobulin-like messenger RNA in a mouse T cell lymphoma.

T H Rabitts, A Forster, M Smith, S Gillam.   

Abstract

RNA-driven complementary DNA (cDNA) hybridization experiments have been carried out in order to detect complementary sequences in RNA prepared from a mouse T cell lymphoma line (EL4). In conditions where efficient hybridization of L-chain cDNA with homologous P3 myeloma mRNA was observed, poor hybridization was observed with EL4 mRNA unless a low criterion of hybrid formation was employed (i.e. hydroxyapatite fractionation). The hybrid formed between EL4 mRNA and L-chain cDNA was found to melt about 5 degrees C below the homologous hybrid indicating that the sequence detected in EL4 mRNA is similar but not identical with the P3 mRNA sequence. However, a similar sequence was detectable in a Clambda-producing myeloma cell line but in this line the concentration of the sequence was found to be an order of magnitude lower than in EL4 cells.

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Year:  1977        PMID： 66149     DOI： 10.1002/eji.1830070111

Source DB:  PubMed          Journal:  Eur J Immunol        ISSN： 0014-2980            Impact factor:   5.532


  6 in total

1.  Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed addition of deoxyribonucleotides to primers which are good or poor acceptors.

Authors:  E M Trip; M Smith
Journal:  Nucleic Acids Res       Date:  1978-05       Impact factor: 16.971

2.  The sequence at the 3' terminus of mouse immunoglobulin secreted mu chain messenger RNA determined from cloned cDNA.

Authors:  G Matthyssens; T H Rabbitts
Journal:  Nucleic Acids Res       Date:  1980-02-25       Impact factor: 16.971

3.  Human immunoglobulin heavy chain genes: evolutionary comparisons of C mu, C delta and C gamma genes and associated switch sequences.

Authors:  T H Rabbitts; A Forster; C P Milstein
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

4.  Expression of the immunoglobulin C mu gene in mouse T and B lymphoid and myeloid cell lines.

Authors:  D J Kemp; A W Harris; S Cory; J M Adams
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

5.  The sequence of a human immunoglobulin epsilon heavy chain constant region gene, and evidence for three non-allelic genes.

Authors:  J G Flanagan; T H Rabbitts
Journal:  EMBO J       Date:  1982       Impact factor: 11.598

6.  Membrane-bound ribosomes of myeloma cells. IV. mRNA complexity of free and membrane-bound polysomes.

Authors:  B Mechler; T H Rabbitts
Journal:  J Cell Biol       Date:  1981-01       Impact factor: 10.539
  6 in total

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