Literature DB >> 6614474

A practical approach for quantitating specific mRNAs by solution hybridization.

D M Durnam, R D Palmiter.   

Abstract

The preparation and use of a specific cDNA probe for quantitating mRNA by solution hybridization is described. Cloned DNA sequences are nick translated, denatured, hybridized to single-stranded M13 clones containing message strand (mDNA) sequences, and separated chromatographically on Bio-Gel A50 under first native and then denaturing conditions to yield a single-stranded cDNA probe. The details of a solution hybridization assay in which the single-stranded cDNA is used to quantitate mRNA in total nucleic acid samples are described. As little as 0.5 pg of mRNA can easily be detected within a day of sample isolation. Thus, the assay is both rapid and sensitive and can be used to measure RNAs complementary to any cloned DNA sequence. It is ideally suited to situations when accurate quantitation of multiple samples is anticipated.

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Year:  1983        PMID： 6614474     DOI： 10.1016/0003-2697(83)90188-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  75 in total

1.  Tissue-specific, developmental, hormonal, and dietary regulation of rat phosphoenolpyruvate carboxykinase-human growth hormone fusion genes in transgenic mice.

Authors:  M K Short; D E Clouthier; I M Schaefer; R E Hammer; M A Magnuson; E G Beale
Journal:  Mol Cell Biol       Date:  1992-03       Impact factor: 4.272

2.  Regulation of muscarinic acetylcholine receptor mRNA expression by activation of homologous and heterologous receptors.

Authors:  B A Habecker; N M Nathanson
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

3.  Insulin-like growth factor I (IGF-I) mRNA expression in coho salmon, Oncorhynchus kisutch: Tissue distribution and effects of growth hormone/prolactin family proteins.

Authors:  C Duan; S J Duguay; E M Plisetskaya
Journal:  Fish Physiol Biochem       Date:  1993-07       Impact factor: 2.794

4.  Epidermal growth factor receptor gene expression and binding capacity in renal cell carcinoma, in relation to tumor stage, grade and DNA ploidy.

Authors:  B Ljungberg; M Gåfvels; J E Damber
Journal:  Urol Res       Date:  1994

5.  Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs.

Authors:  R L Brinster; H Y Chen; M E Trumbauer; M K Yagle; R D Palmiter
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

6.  Rat lung Cu,Zn superoxide dismutase. Isolation and sequence of a full-length cDNA and studies of enzyme induction.

Authors:  M A Hass; J Iqbal; L B Clerch; L Frank; D Massaro
Journal:  J Clin Invest       Date:  1989-04       Impact factor: 14.808

7.  Elimination of zinc from synaptic vesicles in the intact mouse brain by disruption of the ZnT3 gene.

Authors:  T B Cole; H J Wenzel; K E Kafer; P A Schwartzkroin; R D Palmiter
Journal:  Proc Natl Acad Sci U S A       Date:  1999-02-16       Impact factor: 11.205

8.  Induction of mouse metallothionein-I mRNA by bacterial endotoxin is independent of metals and glucocorticoid hormones.

Authors:  D M Durnam; J S Hoffman; C J Quaife; E P Benditt; H Y Chen; R L Brinster; R D Palmiter
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

9.  Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors.

Authors:  R L Brinster; H Y Chen; A Messing; T van Dyke; A J Levine; R D Palmiter
Journal:  Cell       Date:  1984-06       Impact factor: 41.582

10.  Pertussis toxin treatment alters manganese superoxide dismutase activity in lung. Evidence for lung oxygen toxicity in air-breathing rats.

Authors:  L B Clerch; G Neithardt; U Spencer; J A Melendez; G D Massaro; D Massaro
Journal:  J Clin Invest       Date:  1994-06       Impact factor: 14.808
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