| Literature DB >> 6614469 |
R L Stahl, L F Liebes, C M Farber, R Silber.
Abstract
A simple spectrophotometric assay for dehydroascorbate reductase based on the change in absorbance associated with the formation of ascorbic acid is described. Using a partially purified preparation from spinach leaves, the reaction was found to be linear with time and enzyme concentration. The reaction rate determined by this assay correlated well with that obtained by a high-performance liquid chromatography method. Possible advantages over currently available assays as well as potential applications are discussed.Entities:
Mesh：
Substances：
Year: 1983 PMID： 6614469 DOI： 10.1016/0003-2697(83)90180-x
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365