Hematopoietic subpopulations express cross-reactive, lineage-specific molecules detected by monoclonal antibody.

The molecular specificity of a rat anti-mouse monoclonal antibody for cell surface antigens expressed by T- and B-lymphocyte subsets, erythrocytes and polymorphonuclear neutrophils (PMN) was determined. The antibody reacts with B-lymphocyte-associated molecules which migrated as a sharp 48,000 mol. wt band on SDS-PAGE. The antibody reacts with heterogeneous thymocyte and PMN molecules with a predominant mol. wt of 52,000. The same antibody reacts with heat-stable, amphipathic, organic-solvent-soluble erythrocyte molecules of mol. wt 35,000-40,000 present in Folch upper-phase ganglioside fractions, and evidence is presented that the determinant is protein-defined. Thus, a single monoclonal reagent which recognizes distinct, lineage-specific cell surface proteins on erythroid, lymphoid and myeloid elements may be used to probe not only the characteristic patterns of development of these hematopoietic subsets, but also the biochemical functions of the protein antigens themselves. In the case of B- and T-lymphocytes, such functions may extend to involvement in ligand-induced maturation and repertoire selection.