Literature DB >> 6609650

Purification of human factor VII utilizing O-(diethylaminoethyl)-Sephadex and Sulfopropyl-Sephadex chromatography.

L V Rao, S P Bajaj.   

Abstract

A simple procedure for the large scale purification of unactivated human factor VII is described. The initial steps, common to prior purification methods, include adsorption onto barium citrate, ammonium sulfate fractionation, and DEAE-Sephadex chromatography. Factor VII is isolated in pure unactivated form by one additional step, Sulfopropyl-Sephadex chromatography. Ten liters of plasma yields 1.3 mg of protein representing approximately 30% recovery.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6609650     DOI： 10.1016/0003-2697(84)90230-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Activation of factor VII bound to tissue factor: a key early step in the tissue factor pathway of blood coagulation.

Authors:  L V Rao; S I Rapaport
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

2.  Intrinsic versus extrinsic coagulation. Kinetic considerations.

Authors:  B J Warn-Cramer; S P Bajaj
Journal:  Biochem J       Date:  1986-11-01       Impact factor: 3.857

3.  Inhibitor of the factor VIIa-tissue factor complex is reduced in patients with disseminated intravascular coagulation but not in patients with severe hepatocellular disease.

Authors:  M S Bajaj; S V Rana; R B Wysolmerski; S P Bajaj
Journal:  J Clin Invest       Date:  1987-06       Impact factor: 14.808

4.  Tissue factor-factor VIIa-specific up-regulation of IL-8 expression in MDA-MB-231 cells is mediated by PAR-2 and results in increased cell migration.

Authors:  Gertrud M Hjortoe; Lars C Petersen; Tatjana Albrektsen; Brit B Sorensen; Peder L Norby; Samir K Mandal; Usha R Pendurthi; L Vijaya Mohan Rao
Journal:  Blood       Date:  2004-01-08       Impact factor: 22.113

5.  Depletion of extrinsic pathway inhibitor (EPI) sensitizes rabbits to disseminated intravascular coagulation induced with tissue factor: evidence supporting a physiologic role for EPI as a natural anticoagulant.

Authors:  P M Sandset; B J Warn-Cramer; L V Rao; S L Maki; S I Rapaport
Journal:  Proc Natl Acad Sci U S A       Date:  1991-02-01       Impact factor: 11.205

6.  Endothelial cell protein C receptor cellular localization and trafficking: potential functional implications.

Authors:  Ramesh C Nayak; Prosenjit Sen; Samit Ghosh; Ramakrishnan Gopalakrishnan; Charles T Esmon; Usha R Pendurthi; L Vijaya Mohan Rao
Journal:  Blood       Date:  2009-07-08       Impact factor: 22.113

7.  Characterization of three abnormal factor IX variants (Bm Lake Elsinore, Long Beach, and Los Angeles) of hemophilia-B. Evidence for defects affecting the latent catalytic site.

Authors:  P Usharani; B J Warn-Cramer; C K Kasper; S P Bajaj
Journal:  J Clin Invest       Date:  1985-01       Impact factor: 14.808
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.