Literature DB >> 6609209

Intrasplenic primary immunization for the production of monoclonal antibodies.

M Spitz, L Spitz, R Thorpe, E Eugui.   

Abstract

A novel immunization procedure for eliciting monoclonal antibodies ( McAbs ) is described. With intrasplenic inoculation only small amounts of immunogen are required. As little as 20 micrograms of protein antigen or 2.5 X 10(5) cells have been found sufficient to immunize mice or rat spleen cells for the production of specific McAbs . A high proportion of hybridomas secreting McAbs against cell surface antigens and soluble proteins has been obtained with this immunization procedure. The system could facilitate McAb production in many instances in which only small quantities of immunogen are available.

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Year:  1984        PMID： 6609209     DOI： 10.1016/0022-1759(84)90387-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  30 in total

1.  Spectrum of monoclonal antibodies to coxsackievirus B-3 includes type- and group-specific antibodies.

Authors:  S Yagi; D Schnurr; J Lin
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Production of a bi-specific monoclonal antibody recognizing mouse kappa light chains and horseradish peroxidase. Applications in immunoassays.

Authors:  R L Kenigsberg; A C Cuello
Journal:  Histochemistry       Date:  1990

3.  Epitope mapping of the alpha-toxin of Clostridium perfringens.

Authors:  A J Logan; E D Williamson; R W Titball; D A Percival; A D Shuttleworth; J W Conlan; D C Kelly
Journal:  Infect Immun       Date:  1991-12       Impact factor: 3.441

4.  Purification of the N-acetylglucosaminide alpha(1-3/4)fucosyltransferase of human milk.

Authors:  S Eppenberger-Castori; H Lötscher; J Finne
Journal:  Glycoconj J       Date:  1989       Impact factor: 2.916

5.  Anti-idiotypic antibodies to rubella virus.

Authors:  A Nath; B Slagle; J S Wolinsky
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

6.  Production and Characterization of Monoclonal Antibodies against Aspartate Aminotransferase-P1 from Lupin Root Nodules.

Authors:  W. T. Jones; S. D. Jones; D. Harvey; K. R. Rodber; G. B. Ryan; PHS. Reynolds
Journal:  Plant Physiol       Date:  1994-01       Impact factor: 8.340

7.  Characterization and cellular localization by monoclonal antibodies of the 60 kDa mannose specific lectin of human promyelocytic cells, HL60.

Authors:  V Carpentier; C Vassard; C Plessis; G Motta; M Monsigny; A C Roche
Journal:  Glycoconj J       Date:  1994-08       Impact factor: 2.916

8.  The production of a "universal developer" for the immunological detection of human IgG and its application in immunodiagnostics.

Authors:  F M Semenenko; R L Kenigsberg; A C Cuello
Journal:  Histochemistry       Date:  1988

9.  A 22-kd surface antigen detected by monoclonal antibody E 48 is exclusively expressed in stratified squamous and transitional epithelia.

Authors:  J J Quak; A J Balm; G A van Dongen; J G Brakkee; R J Scheper; G B Snow; C J Meijer
Journal:  Am J Pathol       Date:  1990-01       Impact factor: 4.307

10.  Production and immunocytochemical application of a highly sensitive and specific monoclonal antibody against rat dopamine-beta-hydroxylase.

Authors:  I E Mazzoni; E Jaffe; A C Cuello
Journal:  Histochemistry       Date:  1991
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