Literature DB >> 6604046

Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.

J A Trapani, H A Vaughan, R L Sparrow, B D Tait, I F McKenzie.   

Abstract

The production and characterization of a new anti-HLA-B27 monoclonal antibody HLA-ABC-m3 is described. This cytotoxic IgG2a antibody binds protein A and is able to precipitate cell surface molecules of 43,000 and 12,000 daltons corresponding to the HLA heavy chain and beta 2-microglobulin. Population testing revealed that the HLA-ABC-m3 antibody reacted with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and with 5/105 HLA-B27- individuals. These five extra reactions were with individuals expressing the cross-reactive HLA-B7 alloantigen, although the affinity of the monoclonal antibody for B27 heterozygous individuals (approx 10(9) M-1) was tenfold greater than with B7 individuals (approx 10(8) M-1). In addition, HLA-ABC-m3 reactivity segregated with HLA-B27 in two families. This monoclonal antibody should be of value in the investigation of the role of HLA-B27 in disease.

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Year:  1983        PMID： 6604046     DOI： 10.1016/0198-8859(83)90058-7

Source DB:  PubMed          Journal:  Hum Immunol        ISSN： 0198-8859            Impact factor:   2.850


  9 in total

1.  Measurement of HLA class I expression in ankylosing spondylitis.

Authors:  P Creamer; J Edmonds; J Sullivan; S Matthews
Journal:  Ann Rheum Dis       Date:  1992-10       Impact factor: 19.103

2.  Lack of correlation between HLA-B27 like antigenic epitopes on Shigella flexneri and the occurrence of reactive arthritis.

Authors:  C G van Bohemen; A J Nabbe; F C Grumet; J E Landheer; H J Dinant; H C Zanen
Journal:  Clin Exp Immunol       Date:  1986-09       Impact factor: 4.330

3.  Klebsiella 'modifying factor': binding studies with HLA-B27+ and B27- lymphocytes.

Authors:  J A Trapani; I F McKenzie
Journal:  Ann Rheum Dis       Date:  1985-03       Impact factor: 19.103

4.  Status of an unpaired thiol group on the HLA-B27 epitope.

Authors:  L McLean; D Perrett; V R Winrow; J R Archer
Journal:  Clin Exp Immunol       Date:  1989-09       Impact factor: 4.330

5.  HLA-B27 subtypes in the spondarthropathies.

Authors:  I L MacLean; S Iqball; P Woo; A C Keat; R A Hughes; G H Kingsley; S C Knight
Journal:  Clin Exp Immunol       Date:  1993-02       Impact factor: 4.330

6.  Two-dimensional gel analysis demonstrates no structural alteration of HLA-B27 polypeptides between patients with ankylosing spondylitis and healthy individuals.

Authors:  J A Trapani; I D Walker; I F McKenzie
Journal:  Ann Rheum Dis       Date:  1984-04       Impact factor: 19.103

7.  Yersinia enterocolitica serotype O:3 alters the expression of serologic HLA-B27 epitopes on human monocytes.

Authors:  M Wuorela; S Jalkanen; J Kirveskari; P Laitio; K Granfors
Journal:  Infect Immun       Date:  1997-06       Impact factor: 3.441

8.  MHC restriction of synovial fluid lymphocyte responses to the triggering organism in reactive arthritis. Absence of a class I-restricted response.

Authors:  A B Hassell; D Pilling; D Reynolds; P F Life; P A Bacon; J S Gaston
Journal:  Clin Exp Immunol       Date:  1992-06       Impact factor: 4.330

9.  Sulphydryl reactivity of the HLA-B27 epitope: accessibility of the free cysteine studied by flow cytometry.

Authors:  L MacLean; M Macey; M Lowdell; S Badakere; M Whelan; D Perrett; J Archer
Journal:  Ann Rheum Dis       Date:  1992-04       Impact factor: 19.103
  9 in total

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