Literature DB >> 6600779

A sensitive radiometric assay for tryptophan hydroxylase applicable to crude extracts.

S J Beevers, R G Knowles, C I Pogson.   

Abstract

We describe here a simple and convenient method for assay of tryptophan 5-monooxygenase (hydroxylase), applicable to enzyme in all states of purification. It is based on the enzyme-catalysed formation of 5-hydroxy-[4-3H]tryptophan from [5-3H]tryptophan, and the subsequent acid-dependent quantitative release of 3H as 3H2O; unreacted substrate is removed with activated charcoal. The assay is linear with respect to both protein concentration and time, and gives results similar to those in a standard fluorimetric assay.

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Year:  1983        PMID： 6600779     DOI： 10.1111/j.1471-4159.1983.tb08066.x

Source DB:  PubMed          Journal:  J Neurochem        ISSN： 0022-3042            Impact factor:   5.372


  7 in total

1.  Identification of amino-terminal sequences contributing to tryptophan hydroxylase tetramer formation.

Authors:  G J Yohrling; S M Mockus; K E Vrana
Journal:  J Mol Neurosci       Date:  1999-02       Impact factor: 3.444

2.  Effects of neonatal bilateral eye enucleation on postnatal development of the monoamines in posterior thalamus of the rat.

Authors:  M L Vizuete; M Santiago; A J Herrera; J L Venero; A Machado; J Cano
Journal:  J Neural Transm Gen Sect       Date:  1991

3.  Serotonin uptake, storage, and synthesis in an immortalized committed cell line derived from mouse teratocarcinoma.

Authors:  M H Buc-Caron; J M Launay; D Lamblin; O Kellermann
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

4.  A chimeric tyrosine/tryptophan hydroxylase. The tyrosine hydroxylase regulatory domain serves to stabilize enzyme activity.

Authors:  S M Mockus; S C Kumer; K E Vrana
Journal:  J Mol Neurosci       Date:  1997-08       Impact factor: 3.444

5.  Chlamydia pneumoniae encodes a functional aromatic amino acid hydroxylase.

Authors:  Stephanie Abromaitis; P Scott Hefty; Richard S Stephens
Journal:  FEMS Immunol Med Microbiol       Date:  2009-01-09

6.  Alteration of biogenic amines in mouse brain regions by alkylating agents. I. Effects of aflatoxin B1 on brain monoamines concentrations and activities of metabolizing enzymes.

Authors:  S Jayasekara; D B Drown; R A Coulombe; R P Sharma
Journal:  Arch Environ Contam Toxicol       Date:  1989 May-Jun       Impact factor: 2.804

7.  Functional characterization of the S41Y (C2755A) polymorphism of tryptophan hydroxylase 2.

Authors:  Nurgul Carkaci-Salli; Ugur Salli; Izel Tekin; Jeremy A Hengst; Moe K Zhao; T Lee Gilman; Anne M Andrews; Kent E Vrana
Journal:  J Neurochem       Date:  2014-06-28       Impact factor: 5.372
  7 in total

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