Literature DB >> 6592579

Thymine glycol and thymidine glycol in human and rat urine: a possible assay for oxidative DNA damage.

R Cathcart, E Schwiers, R L Saul, B N Ames.   

Abstract

Thymine glycol is a DNA damage product of ionizing radiation and other oxidative mutagens. In an attempt to find a noninvasive assay for oxidative DNA damage in individuals, we have developed an HPLC assay for free thymine glycol and thymidine glycol in urine. Our results indicate that humans excrete about 32 nmol of the two glycols per day. Rats, which have a higher specific metabolic rate and a shorter life span, excrete about 15 times more thymine glycol plus thymidine glycol per kg of body weight than do humans. We present evidence that thymine glycol and thymidine glycol are likely to be derived from repair of oxidized DNA, rather than from alternative sources such as the diet or bacterial flora. This noninvasive assay of DNA oxidation products may allow the direct testing of current theories which relate oxidative metabolism to the processes of aging and cancer in man.

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Year:  1984        PMID： 6592579      PMCID： PMC391764          DOI： 10.1073/pnas.81.18.5633

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

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Authors:  P V Hariharan; P A Cerutti
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205
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  66 in total

1.  Inverse radiation dose-rate effects on somatic and germ-line mutations and DNA damage rates.

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Journal:  Proc Natl Acad Sci U S A       Date:  2000-05-09       Impact factor: 11.205

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9.  Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine as a noninvasive biomarker of oxidative stress.

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Journal:  FASEB J       Date:  2009-12-04       Impact factor: 5.191

10.  Hydrogen peroxide-inducible proteins in Salmonella typhimurium overlap with heat shock and other stress proteins.

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Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205
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