Literature DB >> 6588392

A modified method for extraction and purification of prostaglandins with resin XAD-2.

E Seppälä, O Pora, T Metsä-Ketelä.   

Abstract

Non-ionic resins such as XAD-2 have been widely used as part of routine procedures in the gas chromatographic-mass spectrometric determinations of prostaglandins from aqueous solutions. However, there are reports that XAD-2 cannot be used in connection with radioimmunoassay (RIA), because the column constantly leaks a polymer which almost completely inhibits the antigen-antibody binding. It seems, however, possible to overcome these difficulties by modifying the procedure. The present method can be used with RIA and in combination with further chromatographic purifications for GC-MS determinations.

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Year:  1984        PMID: 6588392     DOI: 10.1016/0262-1746(84)90207-5

Source DB:  PubMed          Journal:  Prostaglandins Leukot Med        ISSN: 0262-1746


  15 in total

1.  Tolfenamic acid inhibits leukotriene synthesis in human granulocytes.

Authors:  J Alanko; E Moilanen; J Opas; H Vapaatalo
Journal:  Agents Actions       Date:  1989-01

2.  Effects of diclofenac, indomethacin, tolfenamic acid and hydrocortisone on prostanoid production in healthy and rheumatic synovial cells.

Authors:  E Moilanen
Journal:  Agents Actions       Date:  1989-03

3.  Evening primrose oil and olive oil in treatment of rheumatoid arthritis.

Authors:  J Jäntti; E Seppälä; H Vapaatalo; H Isomäki
Journal:  Clin Rheumatol       Date:  1989-06       Impact factor: 2.980

4.  Neonatal respiratory distress caused by aspiration of a vaginal tablet containing prostaglandin.

Authors:  S Andersson; R Paetau; M Hallman; H Vapaatalo; O Ylikorkala
Journal:  Br Med J (Clin Res Ed)       Date:  1987-07-04

5.  Orally administered tolfenamic acid inhibits leukotriene synthesis in isolated human peripheral polymorphonuclear leukocytes.

Authors:  E Moilanen; J Alanko; A Juhakoski; H Vapaatalo
Journal:  Agents Actions       Date:  1989-08

6.  Eicosanoid production in rheumatoid synovitis.

Authors:  E Moilanen; J Alanko; M Nissilä; M Hämäläinen; H Isomäki; H Vapaatalo
Journal:  Agents Actions       Date:  1989-11

7.  Comparison of in vitro effects of flunixin and tolfenamic acid on human leukocyte and platelet functions.

Authors:  H Kankaanranta; E Moilanen; H Vapaatalo
Journal:  Inflammation       Date:  1993-08       Impact factor: 4.092

8.  Differences in prostanoid production between healthy and rheumatic synovia in vitro.

Authors:  E Moilanen; E Seppälä; M Nissilä; H Vapaatalo
Journal:  Agents Actions       Date:  1987-02

9.  Comparison of the effects of different anti-inflammatory drugs on synovial fluid prostanoid concentrations in patients with rheumatoid arthritis.

Authors:  E Seppälä; M Nissilä; H Isomäki; P Nuotio; E Nykänen; O Laitinen; H Vapaatalo
Journal:  Clin Rheumatol       Date:  1985-09       Impact factor: 2.980

10.  Plasma levels and urinary excretion of prostaglandins in patients with rheumatoid arthritis.

Authors:  O Laitinen; E Seppalä; M Nissilä; H Vapaatalo
Journal:  Clin Rheumatol       Date:  1983-12       Impact factor: 2.980

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