| Literature DB >> 658779 |
S Iwanaga, T Morita, T Harada, S Nakamura, M Niwa, K Takada, T Kimura, S Sakakibara.
Abstract
An endotoxin-activated hemocyte lysate from the horseshoe crab (Tachypleus and Limulus) was found to hydrolyze Bz-Ile-Glu-(gamma-OR)-Gly-Arg-p-nitroanilide (PNA), Bz-Val-Gly-Arg-PNA, Boc-Val-Leu-Gly-Arg-PNA, and Boc-Leu-Gly-Arg-PNA, all of which have the COOH-terminal Gly-Arg sequence. This amidase activity was due to a clottting enzyme contained in the lysate. Furthermore, the amidase activity increased by increasing the concentration of bacterial endotoxin (E. coli, 0111-B4) added to the lysate. Therefore, the measurement of the endotoxin-induced amidase activity made it possible to determine the concentration of the endotoxin.Entities:
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Year: 1978 PMID： 658779 DOI： 10.1159/000214260
Source DB: PubMed Journal: Haemostasis ISSN： 0301-0147