Literature DB >> 6587322

Elimination of mRNA splicing by a point mutation outside the conserved GU at 5' splice sites.

C Montell, A J Berk.   

Abstract

Nearly all mRNA introns begin with the dinucleotide GU. Mutations in either of these virtually invariant bases have been found to inactivate the corresponding 5' splice site. Until now single base changes in neighboring bases have not been found to completely inactivate a 5' splice site. Here we show that a single A----U transversion in the third position of the adenovirus 2 E1A 13S mRNA intron does prevent RNA splicing at the corresponding 5' splice site.

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Year:  1984        PMID： 6587322      PMCID： PMC318792          DOI： 10.1093/nar/12.9.3821

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  31 in total

1.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

2.  Characteristics of a human cell line transformed by DNA from human adenovirus type 5.

Authors:  F L Graham; J Smiley; W C Russell; R Nairn
Journal:  J Gen Virol       Date:  1977-07       Impact factor: 3.891

3.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

4.  Efficient correction of a mutation by use of chemically synthesized DNA.

Authors:  A Razin; T Hirose; K Itakura; A D Riggs
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

5.  Adenovirus 5 DNA sequences present and RNA sequences transcribed in transformed human embryo kidney cells (HEK-Ad-5 or 293).

Authors:  L Aiello; R Guilfoyle; K Huebner; R Weinmann
Journal:  Virology       Date:  1979-04-30       Impact factor: 3.616

6.  Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.

Authors:  R Breathnach; C Benoist; K O'Hare; F Gannon; P Chambon
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

7.  Complete transformation by adenovirus 2 requires both E1A proteins.

Authors:  C Montell; G Courtois; C Eng; A Berk
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

8.  Mutagenesis at a specific position in a DNA sequence.

Authors:  C A Hutchison; S Phillips; M H Edgell; S Gillam; P Jahnke; M Smith
Journal:  J Biol Chem       Date:  1978-09-25       Impact factor: 5.157

9.  Structure of the adenovirus 2 early mRNAs.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

10.  The primary structure of the duck alpha D-globin gene: an unusual 5' splice junction sequence.

Authors:  C Erbil; J Niessing
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  10 in total

1.  Spectrin Rouen (beta 220-218), a novel shortened beta-chain variant in a kindred with hereditary elliptocytosis. Characterization of the molecular defect as exon skipping due to a splice site mutation.

Authors:  M Garbarz; W T Tse; P G Gallagher; C Picat; M C Lecomte; F Galibert; D Dhermy; B G Forget
Journal:  J Clin Invest       Date:  1991-07       Impact factor: 14.808

2.  Evidence for nuclear factors involved in recognition of 5' splice sites.

Authors:  M L Zapp; S M Berget
Journal:  Nucleic Acids Res       Date:  1989-04-11       Impact factor: 16.971

3.  Splicing of the adenovirus-2 E1A 13S mRNA requires a minimal intron length and specific intron signals.

Authors:  P J Ulfendahl; U Pettersson; G Akusjärvi
Journal:  Nucleic Acids Res       Date:  1985-09-11       Impact factor: 16.971

4.  In vitro splicing of simian virus 40 early pre mRNA.

Authors:  J C Noble; C Prives; J L Manley
Journal:  Nucleic Acids Res       Date:  1986-02-11       Impact factor: 16.971

5.  The 5' splice site: phylogenetic evolution and variable geometry of association with U1RNA.

Authors:  M Jacob; H Gallinaro
Journal:  Nucleic Acids Res       Date:  1989-03-25       Impact factor: 16.971

6.  Recognition of mutant and cryptic 5' splice sites by the U1 small nuclear ribonucleoprotein in vitro.

Authors:  B Chabot; J A Steitz
Journal:  Mol Cell Biol       Date:  1987-02       Impact factor: 4.272

7.  Virus deletion mutants that affect a 3' splice site in the E3 transcription unit of adenovirus 2.

Authors:  B M Bhat; H A Brady; W S Wold
Journal:  Mol Cell Biol       Date:  1985-09       Impact factor: 4.272

8.  Identification of common cystic fibrosis mutations in African-Americans with cystic fibrosis increases the detection rate to 75%.

Authors:  M Macek; A Mackova; A Hamosh; B C Hilman; R F Selden; G Lucotte; K J Friedman; M R Knowles; B J Rosenstein; G R Cutting
Journal:  Am J Hum Genet       Date:  1997-05       Impact factor: 11.025

9.  Two alpha subunit donor splice site mutations cause human trifunctional protein deficiency.

Authors:  J C Brackett; H F Sims; P Rinaldo; S Shapiro; C K Powell; M J Bennett; A W Strauss
Journal:  J Clin Invest       Date:  1995-05       Impact factor: 14.808

10.  Dedifferentiation by adenovirus E1A due to inactivation of Hippo pathway effectors YAP and TAZ.

Authors:  Nathan R Zemke; Dawei Gou; Arnold J Berk
Journal:  Genes Dev       Date:  2019-06-06       Impact factor: 11.361
  10 in total

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