Oxidative burst-dependent tumoricidal and tumorostatic activities of paraffin oil-elicited mouse macrophages.

Mouse peritoneal macrophages (MPM) from C57BL/6J, BALB/c, A strains, and (BALB/ cfemale X C57BL/ 6Jmale )F1 offspring were treated with the oxidative burst (OB)-stimulant 12-O-tetradecanoylphorbol 13-acetate, and their in vitro tumoricidal, tumorostatic , and OB activities were examined. Paraffin oil-elicited, but not thioglycollate (TG)-elicited, MPM exhibited cytotoxicity only toward Yac-1 cells and cytostatic activity toward Yac-1, EL 4, RBL-5, and RLmale 1 lymphoma cells. This activity was in correlation with the reduced capacity of TG-elicited cells to generate OB products. The toxic effect of such activated MPM was partially inhibited by catalase, superoxide dismutase, cytochrome c, and vitamin E (alpha-tocopherol) and was augmented by horseradish peroxidase and the catalase inhibitor aminotriazole (3-amino-1H-1,2,4-triazole), thus indicating the involvement of oxygen-derived toxic reagents, mainly hydrogen peroxide, in the MPM-mediated damage inflicted on the tumor cells. EL 4 cells incubated with nonstimulated MPM exhibited enhanced growth both in vitro and in vivo, whereas OB-stimulated MPM inhibited the growth of such cells in the same experimental systems.