Literature DB >> 6584893

Human dihydrofolate reductase gene is located in chromosome 5 and is unlinked to the related pseudogenes.

B J Maurer, P E Barker, J N Masters, F H Ruddle, G Attardi.   

Abstract

The chromosomal location of the human dihydrofolate reductase (DHFR; EC 1.5.1.3) gene that is amplified in a methotrexate-resistant human cell line has been investigated by screening a large number of human-mouse cell hybrids containing overlapping subsets of human chromosomes. A correlation of genomic blotting data with the chromosome constitution of the individual cell hybrids has allowed the assignment of the human DHFR gene to chromosome 5. This chromosome assignment has been confirmed by the observation of a concomitant loss of the human DHFR gene and of sensitivity to diphtheria toxin, a marker associated with chromosome 5, in two human-mouse cell hybrids selected for resistance to the toxin. Six EcoRI fragments of human DNA containing DHFR pseudogenes or other DHFR-related sequences have been assigned to chromosomes other than chromosome 5.

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Year:  1984        PMID： 6584893      PMCID： PMC344861          DOI： 10.1073/pnas.81.5.1484

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Proceedings: Hexosaminidase B: assignment of a locus involved in its expression to chromosome 5 in man.

Authors:  F Gilbert; R Kucherlapati; R Creagan; M J Murnane; G J Darlington; F H Ruddle
Journal:  Cytogenet Cell Genet       Date:  1974

3.  Multiple forms of human dihydrofolate reductase messenger RNA. Cloning and expression in Escherichia coli of their DNA coding sequence.

Authors:  C Morandi; J N Masters; M Mottes; G Attardi
Journal:  J Mol Biol       Date:  1982-04-15       Impact factor: 5.469

4.  The Chinese hamster gene map. Assignment of four genes (DTS, PGM2, 6PGD, EnoI) to chromosome 2.

Authors:  M Roberts; F H Ruddle
Journal:  Exp Cell Res       Date:  1980-05       Impact factor: 3.905

5.  Isolation of high-molecular-weight DNA from mammalian cells.

Authors:  M Gross-Bellard; P Oudet; P Chambon
Journal:  Eur J Biochem       Date:  1973-07-02

6.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

7.  Variable content of double minute chromosomes is not correlated with degree of phenotype instability in methotrexate-resistant human cell lines.

Authors:  J Masters; B Keeley; H Gay; G Attardi
Journal:  Mol Cell Biol       Date:  1982-05       Impact factor: 4.272

8.  Structure of amplified normal and variant dihydrofolate reductase genes in mouse sarcoma S180 cells.

Authors:  G F Crouse; C C Simonsen; R N McEwan; R T Schimke
Journal:  J Biol Chem       Date:  1982-07-10       Impact factor: 5.157

9.  Intronless human dihydrofolate reductase genes are derived from processed RNA molecules.

Authors:  M J Chen; T Shimada; A D Moulton; M Harrison; A W Nienhuis
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

10.  Genetic analysis of the cell surface: association of human chromosome 5 with sensitivity to diphtheria toxin in mouse-human somatic cell hybrids.

Authors:  R P Creagan; S Chen; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205
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  11 in total

1.  Insight into the molecular mechanism about lowered dihydrofolate binding affinity to dihydrofolate reductase-like 1 (DHFRL1).

Authors:  Jian Gao; Wei Cui; Yuguo Du; Mingjuan Ji
Journal:  J Mol Model       Date:  2013-10-12       Impact factor: 1.810

2.  Murine invariant chain gene: chromosomal assignment and segregation in recombinant inbred strains.

Authors:  J E Richards; D D Pravtcheva; C Day; F H Ruddle; P P Jones
Journal:  Immunogenetics       Date:  1985       Impact factor: 2.846

3.  Chromosomal localization and racial distribution of the polymorphic human dihydrofolate reductase pseudogene (DHFRP1).

Authors:  N P Anagnou; S E Antonarakis; S J O'Brien; W S Modi; A W Nienhuis
Journal:  Am J Hum Genet       Date:  1988-02       Impact factor: 11.025

Review 4.  Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA.

Authors:  M Wagner; R P Perry
Journal:  Mol Cell Biol       Date:  1985-12       Impact factor: 4.272

5.  5' Nucleotide sequences influence serum-modulated expression of a human dihydrofolate reductase minigene.

Authors:  M E Goldsmith; C A Beckman; K H Cowan
Journal:  Mol Cell Biol       Date:  1986-03       Impact factor: 4.272

6.  The former annotated human pseudogene dihydrofolate reductase-like 1 (DHFRL1) is expressed and functional.

Authors:  Gráinne McEntee; Stefano Minguzzi; Kirsty O'Brien; Nadia Ben Larbi; Christine Loscher; Ciarán O'Fágáin; Anne Parle-McDermott
Journal:  Proc Natl Acad Sci U S A       Date:  2011-08-26       Impact factor: 11.205

7.  Chromosomal organization of the human dihydrofolate reductase genes: dispersion, selective amplification, and a novel form of polymorphism.

Authors:  N P Anagnou; S J O'Brien; T Shimada; W G Nash; M J Chen; A W Nienhuis
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

8.  Human genes involved in cholesterol metabolism: chromosomal mapping of the loci for the low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl-coenzyme A reductase with cDNA probes.

Authors:  V Lindgren; K L Luskey; D W Russell; U Francke
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

9.  Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5.

Authors:  V L Funanage; T T Myoda; P A Moses; H R Cowell
Journal:  Mol Cell Biol       Date:  1984-10       Impact factor: 4.272

10.  Early appearance and long-term persistence of the submicroscopic extrachromosomal elements (amplisomes) containing the amplified DHFR genes in human cell lines.

Authors:  G Pauletti; E Lai; G Attardi
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205
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