Literature DB >> 658271

On the solvent-extraction of acetylcholine receptor.

J P Christopher, D B Millar, M A Grafius.   

Abstract

Chloroform/methanol rechromatography on Sephadex LH-20 of fractions isolated from organic solvent extracts of E. electricus electric organs exhibited alterations in elution point compared to the original chromatography. Additionally, 3H decamethonium bromide did not coelute with any of the observed fractions. It is concluded that as presently described the organic solvent isolation procedure for the cholinergic receptor exhibits undesirable characteristics.

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Year:  1978        PMID： 658271     DOI： 10.1007/BF01947259

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  7 in total

1.  Proteolipids from membrane systems.

Authors:  J Folch-Pi; P J Stoffyn
Journal:  Ann N Y Acad Sci       Date:  1972-06-20       Impact factor: 5.691

2.  Use of affinity chromatography for acetylcholine receptor purification.

Authors:  J Schmidt; M A Raftery
Journal:  Biochem Biophys Res Commun       Date:  1972-10-17       Impact factor: 3.575

3.  Isolation of acetylcholine receptors by chloroform--methanol extraction: artifacts arising in use of Sephadex LH-20 columns.

Authors:  S R Levinson; R D Keynes
Journal:  Biochim Biophys Acta       Date:  1972-10-23

4.  Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.

Authors:  S Udenfriend; S Stein; P Böhlen; W Dairman; W Leimgruber; M Weigele
Journal:  Science       Date:  1972-11-24       Impact factor: 47.728

5.  Studies on the cholinergic receptor protein of Electrophorus electricus. I. An assay in vitro for the cholinergic receptor site and solubilization of the receptor protein from electric tissue.

Authors:  J P Changeux; J C Meunier; M Huchet
Journal:  Mol Pharmacol       Date:  1971-09       Impact factor: 4.436

6.  Fractionation of lipids and proteolipids from cat grey white matter by chromatography on an organophilic dextran gel.

Authors:  E F Soto; J M Pasquini; R Plácido; J L La Torre
Journal:  J Chromatogr       Date:  1969-05-20

7.  Multiple binding sites for acetylcholine in a proteolipid from electric tissue.

Authors:  E De Robertis; G S Lunt; J L La Torre
Journal:  Mol Pharmacol       Date:  1971-01       Impact factor: 4.436
  7 in total

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