Literature DB >> 6582493

Purification and characterization of acidic fibroblast growth factor from bovine brain.

K A Thomas, M Rios-Candelore, S Fitzpatrick.   

Abstract

Acidic brain fibroblast growth factor has been purified a minimum of 35,000-fold to apparent homogeneity by a combination of differential salt precipitation, ion exchange chromatography, gel filtration, isoelectric focusing, and hydrophobic chromatography on a C4 reversed-phase HPLC column. Two microheterogeneous forms of the molecule are obtained with apparent molecular masses of 16,600 and 16,800 daltons. The mitogen is highly active with half-maximal stimulation of BALB/c 3T3 fibroblasts at about 40 pg/ml in an assay using incorporation of [methyl-3H]thymidine into DNA.

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Year:  1984        PMID： 6582493      PMCID： PMC344675          DOI： 10.1073/pnas.81.2.357

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  Platelet-derived growth factor: purification and partial characterization.

Authors:  C H Heldin; B Westermark; A Wasteson
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

2.  Epidermal growth factor. Physical and chemical properties.

Authors:  J M Taylor; W M Mitchell; S Cohen
Journal:  J Biol Chem       Date:  1972-09-25       Impact factor: 5.157

3.  Purification and partial characterization of bovine pituitary fibroblast growth factor.

Authors:  S K Lemmon; R A Bradshaw
Journal:  J Cell Biochem       Date:  1983       Impact factor: 4.429

4.  Purification of the fibroblast growth factor activity from bovine brain.

Authors:  D Gospodarowicz; H Bialecki; G Greenburg
Journal:  J Biol Chem       Date:  1978-05-25       Impact factor: 5.157

5.  Primary structure of human insulin-like growth factor II.

Authors:  E Rinderknecht; R E Humbel
Journal:  FEBS Lett       Date:  1978-05-15       Impact factor: 4.124

6.  Complete amino acid analysis of proteins from a single hydrolysate.

Authors:  R J Simpson; M R Neuberger; T Y Liu
Journal:  J Biol Chem       Date:  1976-04-10       Impact factor: 5.157

7.  Brain fibroblast growth factor: nonidentity with myelin basic protein fragments.

Authors:  K A Thomas; M C Riley; S K Lemmon; N C Baglan; R A Bradshaw
Journal:  J Biol Chem       Date:  1980-06-25       Impact factor: 5.157

8.  Brain-derived fibroblast growth factor: identity with a fragment of the basic protein of myelin.

Authors:  F C Westall; V A Lennon; D Gospodarowicz
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

9.  Polypeptides with nonsuppressible insulin-like and cell-growth promoting activities in human serum: isolation, chemical characterization, and some biological properties of forms I and II.

Authors:  E Rinderknecht; R E Humbel
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

10.  The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin.

Authors:  E Rinderknecht; R E Humbel
Journal:  J Biol Chem       Date:  1978-04-25       Impact factor: 5.157
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  47 in total

1.  Heparin-binding growth factors and their receptors.

Authors:  B B Olwin
Journal:  Cytotechnology       Date:  1989-12       Impact factor: 2.058

2.  Pure brain-derived acidic fibroblast growth factor is a potent angiogenic vascular endothelial cell mitogen with sequence homology to interleukin 1.

Authors:  K A Thomas; M Rios-Candelore; G Giménez-Gallego; J DiSalvo; C Bennett; J Rodkey; S Fitzpatrick
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

3.  Purification of a glycoprotein vascular endothelial cell mitogen from a rat glioma-derived cell line.

Authors:  G Conn; D D Soderman; M T Schaeffer; M Wile; V B Hatcher; K A Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

Review 4.  Fibroblast growth factor regulation of neovascularization.

Authors:  Masahiro Murakami; Michael Simons
Journal:  Curr Opin Hematol       Date:  2008-05       Impact factor: 3.284

Review 5.  New aspects of placental endocrinology.

Authors:  F Petraglia; L Calzà; G C Garuti; L Giardino; B M De Ramundo; S Angioni
Journal:  J Endocrinol Invest       Date:  1990-04       Impact factor: 4.256

6.  Evidence and characterization of the receptor to eye-derived growth factor I, the retinal form of basic fibroblast growth factor, on bovine epithelial lens cells.

Authors:  M Moenner; B Chevallier; J Badet; D Barritault
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

7.  Growth of MTW9/PL2 estrogen-responsive rat mammary tumor cells in hormonally defined serum-free media.

Authors:  D Danielpour; T L Riss; M Ogasawara; D A Sirbasku
Journal:  In Vitro Cell Dev Biol       Date:  1988-01

8.  Species-specific differences in the mitogenic activity of heparin-binding growth factors in the sera of various mammals.

Authors:  Y Yonezawa; H Kondo; R Hirai; K Kaji; K Nishikawa
Journal:  Experientia       Date:  1992-03-15

9.  Astroglial and fibroblast growth factors have neurotrophic functions for cultured peripheral and central nervous system neurons.

Authors:  K Unsicker; H Reichert-Preibsch; R Schmidt; B Pettmann; G Labourdette; M Sensenbrenner
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

10.  Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos.

Authors:  K Inaba; M Seno; H Morino
Journal:  Fish Physiol Biochem       Date:  1996-08       Impact factor: 2.794
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