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Literature DB >> 658229

Metal binding to myosin and to myosin DTNB-light chain.

Abstract

The effects of various divalent cations, Ca2+, Mg2+ and Mn2+ on the intrinsic fluorescence of heavy meromyosin (HMM) and myosin 5,5'-dithio-bis-(2-nitrobenzoate) DTNB-light chain of rabbit striated muscle, are compared. At pH 6.4, th fluorescence change induced by the metal ions is present only in the isolated light chain and disappears in HMM, thus indicating an interaction between the heavy and light chains with respect to the binding of the metal ions. Whereas Mg2+ binds more strongly than Ca2+ to myosin, this order is reversed in the case of the DTNB-light chain.

Entities: Chemical Species

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Year: 1978 PMID： 658229 DOI： 10.1007/BF01936967

Source DB: PubMed Journal: Experientia ISSN： 0014-4754

21 in total

1. Comparison of the calcium sensitivity of actomyosin from native and L-2-deficient myosin.

Authors: S M Pemrick
Journal: Biochemistry Date: 1977-09-06 Impact factor: 3.162

2. Calcium ion-dependent myosin from decapod-crustacean muscles.

Authors: W Lehman
Journal: Biochem J Date: 1977-05-01 Impact factor: 3.857

3. Calcium binding to rabbit skeletal myosin under physiological conditions.

Authors: R D Bremel; A Weber
Journal: Biochim Biophys Acta Date: 1975-02-17

4. Myosin-linked calcium regulation in vertebrate smooth muscle.

Authors: A Sobieszek; J V Small
Journal: J Mol Biol Date: 1976-03-25 Impact factor: 5.469

5. Studies on the chymotryptic digestion of myosin. Effects of divalent cations on proteolytic susceptibility.

Authors: A G Weeds; B Pope
Journal: J Mol Biol Date: 1977-04 Impact factor: 5.469

6. Evidence for structural changes in vertebrate thick filaments induced by calcium.

Authors: K Morimoto; W F Harrington
Journal: J Mol Biol Date: 1974-09-25 Impact factor: 5.469

7. Physico-chemical studies on the light chains of myosin. 3. Evidence for a regulatory role of a rabbit myosin light chain.

Authors: M M Werber; A Oplatka
Journal: Biochem Biophys Res Commun Date: 1974-04-08 Impact factor: 3.575

Metal binding to myosin and to myosin DTNB-light chain.

1. Comparison of the calcium sensitivity of actomyosin from native and L-2-deficient myosin.

2. Calcium ion-dependent myosin from decapod-crustacean muscles.

3. Calcium binding to rabbit skeletal myosin under physiological conditions.

4. Myosin-linked calcium regulation in vertebrate smooth muscle.

5. Studies on the chymotryptic digestion of myosin. Effects of divalent cations on proteolytic susceptibility.

6. Evidence for structural changes in vertebrate thick filaments induced by calcium.

7. Physico-chemical studies on the light chains of myosin. 3. Evidence for a regulatory role of a rabbit myosin light chain.

8. Studies on the low molecular weight protein of myosin.

9. Fluorescence studies on the interaction between heavy meromyosin and magnesium ions. Effect of EDTA.

10. On the location of the divalent metal binding sites and the light chain subunits of vertebrate myosin.

1. Properties of the non-specific calcium-binding sites of rabbit skeletal-muscle myosin.

2. Influence of myosin heavy chains on the Ca2+-binding properties of light chain, LC2.