Literature DB >> 6577259

Methods utilizing cell-free protein-synthesizing systems for the identification of recombinant DNA molecules.

J S Miller, B M Paterson, R P Ricciardi, L Cohen, B E Roberts.   

Abstract

Herein we outline three methods that, when coupled with cell-free protein synthesis, permit the identification of recombinant DNA molecules encoding specific polypeptides. RNAs enriched by fractionation on methylmercury hydroxide agarose gels are used to prepare sequence-specific probes. Recombinant DNA clones thus identified are further characterized as to their encoded polypeptides by either hybrid selection or hybrid arrest of translation.

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Year:  1983        PMID： 6577259     DOI： 10.1016/0076-6879(83)01046-0

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  28 in total

1.  Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene.

Authors:  B A Morris-Krsinich; P M Mullineaux; J Donson; M I Boulton; P G Markham; M N Short; J W Davies
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

2.  Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris.

Authors:  K E Broglie; J J Gaynor; R M Broglie
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

3.  Coordinate regulation of stromelysin and collagenase genes determined with cDNA probes.

Authors:  S M Frisch; E J Clark; Z Werb
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

4.  In vitro expression of two proteins from overlapping reading frames in a eukaryotic DNA sequence.

Authors:  J M Jankowski; S A Krawetz; E Walczyk; G H Dixon
Journal:  J Mol Evol       Date:  1986       Impact factor: 2.395

5.  Molecular genetic analysis of a vaccinia virus gene with an essential role in DNA replication.

Authors:  E Evans; P Traktman
Journal:  J Virol       Date:  1987-10       Impact factor: 5.103

6.  Isolation and characterization of a cDNA clone for the Cat2 gene in maize and its homology with other catalases.

Authors:  L A Bethards; R W Skadsen; J G Scandalios
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

7.  The use of single-stranded DNA and RNase H to promote quantitative 'hybrid arrest of translation' of mRNA/DNA hybrids in reticulocyte lysate cell-free translations.

Authors:  J Minshull; T Hunt
Journal:  Nucleic Acids Res       Date:  1986-08-26       Impact factor: 16.971

8.  Isolation and characterization of a cDNA clone encoding avian skeletal muscle C-protein: an intracellular member of the immunoglobulin superfamily.

Authors:  S Einheber; D A Fischman
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

9.  Transcriptional mapping of the amplified region encoding the dihydrofolate reductase-thymidylate synthase of Leishmania major reveals a high density of transcripts, including overlapping and antisense RNAs.

Authors:  G M Kapler; S M Beverley
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

10.  Temperature-sensitive vaccinia virus mutants identify a gene with an essential role in viral replication.

Authors:  R E Rempel; M K Anderson; E Evans; P Traktman
Journal:  J Virol       Date:  1990-02       Impact factor: 5.103
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