| Literature DB >> 6575973 |
P R Clements, D J Mahuran, J J Hopwood.
Abstract
Elution of bound glycoproteins from concanavalin A-Sepharose can be made more efficient by their readsorption to a Blue A agarose column (specific) and Green A agarose column (less-specific) during recycling of the elution buffer. Three lysosomal enzymes were eluted in this way with marked improvement in their specific activities, time and handling and amount of eluting ligand used.Entities:
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Year: 1983 PMID： 6575973 DOI： 10.1016/s0021-9673(01)87920-6
Source DB: PubMed Journal: J Chromatogr