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Literature DB >> 6574481

Simplified in vitro system for study of eukaryotic mRNA translation by measuring di- and tripeptide formation.

Y Cenatiempo, T Twardowski, B Redfield, B R Reid, H Dauerman, H Weissbach, N Brot.

Abstract

An in vitro system for measurement of rabbit globin mRNA translation has been developed based on the formation of the NH2-terminal dipeptide, fMet-Val. The basic components include a partially purified initiation factor preparation from rabbit reticulocytes supplemented with eukaryotic initiation factor 4A, purified and formylated yeast Met-tRNAi, and rabbit liver or Escherichia coli Val-tRNA1Val. Picomole quantities of fMet-Val are synthesized, dependent on mRNA, and the dipeptide is readily assayed by a simple extraction procedure. In the presence of Leu-tRNA or His-tRNA, the tripeptides fMet-Val-Leu and fMet-Val-His are synthesized, corresponding to the NH2-terminal sequence of alpha- and beta-globin, respectively. Therefore, tripeptide synthesis provides a simple means to distinguish between the expression of the alpha- and beta-globin mRNA species.

Entities: Chemical Gene Species

Mesh：

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Year: 1983 PMID： 6574481 PMCID： PMC394012 DOI： 10.1073/pnas.80.11.3223

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

21 in total

1. Selective utilization of valyl-tRNA having a particular coding specificity in a rabbit hemoglobin synthesizing system.

Authors: K Takeishi; T Takemoto; S Nishimura; T Ukita
Journal: Biochem Biophys Res Commun Date: 1972-05-26 Impact factor: 3.575

2. Model for the regulation of mRNA translation applied to haemoglobin synthesis.

Authors: H F Lodish
Journal: Nature Date: 1974-10-04 Impact factor: 49.962

3. Purification of five serine transfer ribonucleic acid species from Escherichia coli and their acylation by homologous and heterologous seryl transfer ribonucleic acid synthetases.

Authors: K L Roy; D Söll
Journal: J Biol Chem Date: 1970-03-25 Impact factor: 5.157

4. Initiation of globin synthesis. Preparation and use of reticulocyte ribosomes retaining initiation region messenger ribonucleic acid fragments.

Authors: R G Crystal; A W Nienhuis; N A Elson; W F Anderson
Journal: J Biol Chem Date: 1972-09-10 Impact factor: 5.157

5. Alpha and beta globin messenger ribonucleic acid. Different amounts and rates of initiation of translation.

Authors: H F Lodish
Journal: J Biol Chem Date: 1971-12-10 Impact factor: 5.157

6. Methionyl soluble ribonucleic acid transformylase. I. Purification and partial characterization.

Authors: H W Dickerman; E Steers; B G Redfield; H Weissbach
Journal: J Biol Chem Date: 1967-04-10 Impact factor: 5.157

7. Initial dipeptide formation in hemoglobin biosynthesis.

Authors: R G Crystal; D A Shafritz; P M Prichard; W F Anderson
Journal: Proc Natl Acad Sci U S A Date: 1971-08 Impact factor: 11.205

8. Regulation of hemoglobin synthesis. Equal rates of translation and termination of - and -globin chains.

Authors: H F Lodish; M Jacobsen
Journal: J Biol Chem Date: 1972-06-10 Impact factor: 5.157

9. Initiation of hemoglobin synthesis: comparison of model reactions that use artificial templates with those using natural messenger RNA.

Authors: R G Crystal; W F Anderson
Journal: Proc Natl Acad Sci U S A Date: 1972-03 Impact factor: 11.205

5. Posttranscriptional control of embryonic rat skeletal muscle protein synthesis. Control at the level of translation by endogenous RNA.

Authors: C R Vanderburg; M A Nathanson
Journal: J Cell Biol Date: 1988-09 Impact factor: 10.539

5 in total

Simplified in vitro system for study of eukaryotic mRNA translation by measuring di- and tripeptide formation.

1. Selective utilization of valyl-tRNA having a particular coding specificity in a rabbit hemoglobin synthesizing system.

2. Model for the regulation of mRNA translation applied to haemoglobin synthesis.

3. Purification of five serine transfer ribonucleic acid species from Escherichia coli and their acylation by homologous and heterologous seryl transfer ribonucleic acid synthetases.

4. Initiation of globin synthesis. Preparation and use of reticulocyte ribosomes retaining initiation region messenger ribonucleic acid fragments.

5. Alpha and beta globin messenger ribonucleic acid. Different amounts and rates of initiation of translation.

6. Methionyl soluble ribonucleic acid transformylase. I. Purification and partial characterization.

7. Initial dipeptide formation in hemoglobin biosynthesis.

8. Regulation of hemoglobin synthesis. Equal rates of translation and termination of - and -globin chains.

9. Initiation of hemoglobin synthesis: comparison of model reactions that use artificial templates with those using natural messenger RNA.

10. A supernatant factor involved in initiation complex formation with eukaryotic ribosomes.

1. Determination of the translation start site of the large subunit of ribulose-1,5-bisphosphate carboxylase from maize.

2. Identification and chemical synthesis of a ribosomal protein antigenic determinant in systemic lupus erythematosus.

3. Two initiation sites detected in the small s1 species of reovirus mRNA by dipeptide synthesis in vitro.

Review 4. Cell-free protein synthesis: the state of the art.

5. Posttranscriptional control of embryonic rat skeletal muscle protein synthesis. Control at the level of translation by endogenous RNA.