Literature DB >> 6571989

Metal and sulfur composition of iron-molybdenum cofactor of nitrogenase.

M J Nelson, M A Levy, W H Orme-Johnson.   

Abstract

The sulfur content of N-methylformamide solutions of cofactor from Clostridium pasteurianum nitrogenase has been determined to be 11.9 (+/- 0.9) mol per mol of molybdenum. This number was determined radiochemically, using iron-molybdenum cofactor isolated from molybdenum-iron protein from bacteria grown on 35SO4. A total of 3.2 (+/- 0.2) mol of sulfur per mol of molybdenum was found to be present in cysteine and methionine, probably arising from contaminating proteins not intrinsic to the cofactor. Combined with accumulated evidence that is discussed, these results lead to an updated stoichiometry of MoFe6S8 or 9, not MoFe6S4 as previously thought, for this cluster.

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Year:  1983        PMID： 6571989      PMCID： PMC393327          DOI： 10.1073/pnas.80.1.147

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Efficient fluorography of 3H and 14C on thin layers.

Authors:  W M Bonner; J D Stedman
Journal:  Anal Biochem       Date:  1978-08-15       Impact factor: 3.365

2.  Nitrogenase. VIII. Mössbauer and EPR spectroscopy. The MoFe protein component from Azotobacter vinelandii OP.

Authors:  E Münck; H Rhodes; W H Orme-Johnson; L C Davis; W J Brill; V K Shah
Journal:  Biochim Biophys Acta       Date:  1975-07-21

3.  Novel metal cluster in the iron-molybdenum cofactor of nitrogenase. Spectroscopic evidence.

Authors:  J Rawlings; V K Shah; J R Chisnell; W J Brill; R Zimmermann; E Münck; W H Orme-Johnson
Journal:  J Biol Chem       Date:  1978-02-25       Impact factor: 5.157

4.  Structural investigation of nitrogenase components from Clostridium pasteurianum and comparison with similar components of other organisms.

Authors:  J S Chen; J S Multani; L E Mortenson
Journal:  Biochim Biophys Acta       Date:  1973-05-17

5.  Preparation and properties of clostridial ferredoxins.

Authors:  J Rabinowitz
Journal:  Methods Enzymol       Date:  1972       Impact factor: 1.600

6.  Purification of nitrogenase from Clostridium pasteurianum.

Authors:  L E Mortenson
Journal:  Methods Enzymol       Date:  1972       Impact factor: 1.600

7.  The amino acid sequence of Clostridium pasteurianum iron protein, a component of nitrogenase. I. Tryptic peptides.

Authors:  M Tanaka; M Haniu; K T Yasunobu
Journal:  J Biol Chem       Date:  1977-10-25       Impact factor: 5.157

8.  Nitrogenase XI: Mössbauer studies on the cofactor centers of the MoFe protein from Azotobacter vinelandii OP.

Authors:  B H Huynh; E Münck; W H Orme-Johnson
Journal:  Biochim Biophys Acta       Date:  1979-01-25

9.  Isolation of thiomolybdate compounds from the molybdenum-iron protein of clostridial nitrogenase.

Authors:  W G Zumft
Journal:  Eur J Biochem       Date:  1978-11-15

10.  Isolation of an iron-molybdenum cofactor from nitrogenase.

Authors:  V K Shah; W J Brill
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
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  8 in total

1.  Purification and characterization of the nifN and nifE gene products from Azotobacter vinelandii mutant UW45.

Authors:  T D Paustian; V K Shah; G P Roberts
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

2.  Plausible structure of the iron-molybdenum cofactor of nitrogenase.

Authors:  M S Madden; A M Krezel; R M Allen; P W Ludden; V K Shah
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

3.  The inactive MoFe protein (NifB-Kp1) of the nitrogenase from nifB mutants of Klebsiella pneumoniae. Its interaction with FeMo-cofactor and the properties of the active MoFe protein formed.

Authors:  T R Hawkes; B E Smith
Journal:  Biochem J       Date:  1984-11-01       Impact factor: 3.857

4.  Sequence of the nifD gene coding for the alpha subunit of dinitrogenase from the cyanobacterium Anabaena.

Authors:  P J Lammers; R Haselkorn
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205

5.  The N-terminal and C-terminal portions of NifV are encoded by two different genes in Clostridium pasteurianum.

Authors:  S Z Wang; D R Dean; J S Chen; J L Johnson
Journal:  J Bacteriol       Date:  1991-05       Impact factor: 3.490

6.  Nitrogenase from nifV mutants of Klebsiella pneumoniae contains an altered form of the iron-molybdenum cofactor.

Authors:  T R Hawkes; P A McLean; B E Smith
Journal:  Biochem J       Date:  1984-01-01       Impact factor: 3.857

7.  Structural analysis of the genes encoding the molybdenum-iron protein of nitrogenase in the Parasponia rhizobium strain ANU289.

Authors:  J J Weinman; F F Fellows; P M Gresshoff; J Shine; K F Scott
Journal:  Nucleic Acids Res       Date:  1984-11-26       Impact factor: 16.971

8.  The vanadium-iron protein of vanadium nitrogenase from Azotobacter chroococcum contains an iron-vanadium cofactor.

Authors:  B E Smith; R R Eady; D J Lowe; C Gormal
Journal:  Biochem J       Date:  1988-02-15       Impact factor: 3.857
  8 in total

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