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Literature DB >> 6561956

Adsorbed soluble [3H]elastin as substrate for proteinase activity. A new microassay technique.

Abstract

A microassay for elastase activity has been designed. Microtubes are coated with soluble elastin substrate by passive adsorption to the tube walls. On contact with enzymes in test samples, radioactivity is released into the sample buffer, which is then transferred to vials containing scintillation fluid without further processing. Porcine pancreatic elastase (EC 3.4.21.11) is easily detected in concentrations down to 8 micrograms/litre. The interassay coefficient of variation (CV) was below 10% for values above 50 micrograms/litre. The assay is rapid, precise, economical and sensitive.

Entities: Chemical Disease Gene

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Year: 1984 PMID： 6561956 PMCID： PMC1153386 DOI： 10.1042/bj2180645

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

6 in total

1. A new radioactive assay for enzymes with elastolytic activity using reduced tritiated elastin. The effect of sodium dodecyl sulfate on elastolysis.

Authors: S Takahashi; S Seifter; F C Yang
Journal: Biochim Biophys Acta Date: 1973-11-15

2. Amorphous [14C]elastin as a substrate for assaying elastolytic enzyme in cellular and tissue extracts.

Authors: S Y Yu; A Yoshida
Journal: Biochem Med Date: 1979-02

3. Primary enzyme quantitation using substrates labeled with a second indicator enzyme. I. Elastase determination using peroxidase-labeled elastin.

Authors: G C Saunders; Z Svitra; A Martinez
Journal: Anal Biochem Date: 1982-10 Impact factor: 3.365

1 in total

1. Prevention of tissue damage: inhibition of myeloperoxidase mediated inactivation of alpha 1-proteinase inhibitor by N-acetyl cysteine, glutathione, and methionine.

Authors: N Borregaard; H S Jensen; O W Bjerrum
Journal: Agents Actions Date: 1987-12

1 in total

Adsorbed soluble [3H]elastin as substrate for proteinase activity. A new microassay technique.

1. A new radioactive assay for enzymes with elastolytic activity using reduced tritiated elastin. The effect of sodium dodecyl sulfate on elastolysis.

2. Amorphous [14C]elastin as a substrate for assaying elastolytic enzyme in cellular and tissue extracts.

3. Primary enzyme quantitation using substrates labeled with a second indicator enzyme. I. Elastase determination using peroxidase-labeled elastin.

Review 4. Elastin structure, biosynthesis, and relation to disease states.

5. An evaluation of four methods for the measurement of elastase activity.

6. A sensitive and specific enzyme assay for elastase activity using alpha-[3H]elastin as substrate.

1. Prevention of tissue damage: inhibition of myeloperoxidase mediated inactivation of alpha 1-proteinase inhibitor by N-acetyl cysteine, glutathione, and methionine.