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Literature DB >> 6561234

Characterization of three highly purified influenza virus strains by electron microscopy.

R W Ruigrok, P J Andree, R A Hooft van Huysduynen, J E Mellema.

Abstract

Mass determinations on highly purified influenza virus preparations were performed using the technique of scanning transmission electron microscopy. The masses of the three strains, X49, B/Singapore/222/79 and B/Hong Kong/8/73 were determined. The average value was 174 X 10(6) daltons with only small differences between the three strains. The mass of virus particles after removal of the protruding spike proteins, haemagglutinin and neuraminidase by bromelain treatment was determined to be 86 X 10(6) daltons. From the mass difference and the known molecular weight of the spike proteins the number of spikes was estimated to lie in the range 400 to 500.

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Year: 1984 PMID： 6561234 DOI： 10.1099/0022-1317-65-4-799

Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891

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Cited

17 in total

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Journal: Biophys J Date: 2002-11 Impact factor: 4.033

2. Stability of influenza vaccine coated onto microneedles.

Authors: Hyo-Jick Choi; Dae-Goon Yoo; Brian J Bondy; Fu-Shi Quan; Richard W Compans; Sang-Moo Kang; Mark R Prausnitz
Journal: Biomaterials Date: 2012-02-21 Impact factor: 12.479

3. A negative stain for electron microscopic tomography.

Authors: Andrea Fera; Jane E Farrington; Joshua Zimmerberg; Thomas S Reese
Journal: Microsc Microanal Date: 2012-02-27 Impact factor: 4.127

4. Stochastic fusion simulations and experiments suggest passive and active roles of hemagglutinin during membrane fusion.

Authors: Donald W Lee; Vikram Thapar; Paulette Clancy; Susan Daniel
Journal: Biophys J Date: 2014-02-18 Impact factor: 4.033

Characterization of three highly purified influenza virus strains by electron microscopy.

1. Measuring pKa of activation and pKi of inactivation for influenza hemagglutinin from kinetics of membrane fusion of virions and of HA expressing cells.

2. Stability of influenza vaccine coated onto microneedles.

3. A negative stain for electron microscopic tomography.

4. Stochastic fusion simulations and experiments suggest passive and active roles of hemagglutinin during membrane fusion.

5. Mass and molecular composition of vesicular stomatitis virus: a scanning transmission electron microscopy analysis.

6. Structure of clathrin-coated vesicles from small-angle scattering experiments.

7. Labeling of influenza viruses with synthetic fluorescent and biotin-labeled lipids.

8. Dissection of Epitope-Specific Mechanisms of Neutralization of Influenza Virus by Intact IgG and Fab Fragments.

9. Architecture of a nascent viral fusion pore.

10. Ultrasensitive detection of influenza viruses with a glycan-based impedimetric biosensor.