Literature DB >> 6559483

Antibody directed to a synthetic peptide encoding the NH2-terminal 16 amino acids of the adenovirus type 2 E1B-53K tumor antigen recognizes the E1B-20K tumor antigen.

L A Lucher, K H Brackmann, J S Symington, M Green.   

Abstract

A peptide, H2N-Glu-Arg-Arg-Asn-Pro-Ser-Glu-Arg-Gly-Val-Pro-Ala-Gly-Phe-Ser-Gly-(Cys )COOH, containing the amino acid sequence at the NH2 terminus of the adenovirus type 2 (Ad2) E1B-coded large T antigen (E1B-53K) has been synthesized. Anti-peptide antibody was generated in rabbits and used to immunoprecipitate Ad T antigens from Ad2 early infected cell extracts. In addition to the expected E1B-53K T antigen, anti-peptide antibody precipitated the Ad2 E1B-20K T antigen that was previously shown to be related to E1B-53K (M. Green, K.H. Brackmann, M.A. Cartas, and T. Matsuo, J. Virol. 42, 30-41, 1982). Anti-peptide prepared against the COOH terminus of the E1B-53K T antigen or against the NH2 terminus of the E1B-19K T antigen did not precipitate the E1B-20K T antigen. These data suggest that the Ad2 E1B-20K T antigen initiates translation at nucleotide 2016 in reading frame 3, as does E1B-53K. The viral mRNA that encodes the E1B-20K T antigen has not been identified.

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Year:  1984        PMID： 6559483     DOI： 10.1016/0042-6822(84)90106-5

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  9 in total

1.  Identification of adenovirus type 2 early region 1B proteins that share the same amino terminus as do the 495R and 155R proteins.

Authors:  J B Lewis; C W Anderson
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103

Review 2.  The structure and functions of the adenovirus early region 1 proteins.

Authors:  R J Grand
Journal:  Biochem J       Date:  1987-01-01       Impact factor: 3.857

3.  Adenovirus type 5 early region 1B 156R protein promotes cell transformation independently of repression of p53-stimulated transcription.

Authors:  Timo Sieber; Thomas Dobner
Journal:  J Virol       Date:  2006-10-18       Impact factor: 5.103

4.  The adenovirus E1B 19-kilodalton protein stimulates gene expression by increasing DNA levels.

Authors:  C H Herrmann; M B Mathews
Journal:  Mol Cell Biol       Date:  1989-12       Impact factor: 4.272

5.  Regulation of p53-dependent apoptosis, transcriptional repression, and cell transformation by phosphorylation of the 55-kilodalton E1B protein of human adenovirus type 5.

Authors:  J G Teodoro; P E Branton
Journal:  J Virol       Date:  1997-05       Impact factor: 5.103

6.  Organization of early region 1B of human adenovirus type 2: identification of four differentially spliced mRNAs.

Authors:  A Virtanen; U Pettersson
Journal:  J Virol       Date:  1985-05       Impact factor: 5.103

7.  Phosphorylation at the carboxy terminus of the 55-kilodalton adenovirus type 5 E1B protein regulates transforming activity.

Authors:  J G Teodoro; T Halliday; S G Whalen; D Takayesu; F L Graham; P E Branton
Journal:  J Virol       Date:  1994-02       Impact factor: 5.103

8.  Mutations in the gene encoding the adenovirus early region 1B 19,000-molecular-weight tumor antigen cause the degradation of chromosomal DNA.

Authors:  E White; T Grodzicker; B W Stillman
Journal:  J Virol       Date:  1984-11       Impact factor: 5.103

9.  Activation of adenovirus 5 E1A transcription by region E1B in transformed primary rat cells.

Authors:  A G Jochemsen; L T Peltenburg; M F te Pas; C M de Wit; J L Bos; A J van der Eb
Journal:  EMBO J       Date:  1987-11       Impact factor: 11.598
  9 in total

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