| Literature DB >> 6548931 |
S Takeshita, T Aoki, Y Fukumaki, Y Takagi.
Abstract
A cDNA for alpha-globin mRNA of the carp, Cyprinus carpio, was cloned by the method of Okayama and Berg (Mol. Cell. Biol. 2 (1982) 161-170) and its complete nucleotide sequence was determined. The 5' non-coding region contained 23 nucleotides. Following this region, there was an open reading frame encoded with an alpha-globin polypeptide consisting of 142 amino acids. The 3' non-coding region was 88 nucleotides in length, including two copies of the hexanucleotide AATAAA and a poly(A) site of the GC dinucleotide. There were 16 discrepancies between the reported amino acid sequence of the carp alpha-globin chain and the amino acid sequence predicted from the DNA sequence of the clone. The possible explanations for these differences in amino acid sequence are discussed.Entities:
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Year: 1984 PMID： 6548931 DOI： 10.1016/0167-4781(84)90037-x
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002