Literature DB >> 6548571

Detection of the messenger RNA coding for preproenkephalin A in bovine adrenal by in situ hybridization.

B Bloch, R J Milner, A Baird, U Gubler, C Reymond, P Bohlen, D le Guellec, F E Bloom.   

Abstract

The messenger RNA (mRNA) coding for the adrenal precursor of enkephalins (preproenkephalin-A) has been detected in bovine adrenal medulla cells using in situ hybridization with 32P-labelled preproenkephalin A (PPA) complementary DNA. In formaldehyde- and Carnoy-fixed tissue sections, an intense elective labelling restricted to the cells located at the periphery of the adrenal medulla can be detected after hybridization procedure, using X-ray film and classical autoradiographic procedure. Adequate controls show that this labelling is obtained only using PPA complementary DNA, inserted or not in its vector. Distribution of PPA mRNA appears identical to that of its immunoreactive end products, namely Met-enkephalin and BAM22 peptide, detected by immunohistochemistry. Norepinephrine, detectable using monoamine histofluorescence, appears restricted to the cells of the center of the gland unlabelled for PPA mRNA and its end-products. Cultured bovine adrenomedullary cells that exhibited enkephalin immunoreactivity also contain PPA mRNA located in their cytoplasm.

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Year:  1984        PMID： 6548571     DOI： 10.1016/0167-0115(84)90045-4

Source DB:  PubMed          Journal:  Regul Pept        ISSN： 0167-0115


  8 in total

Review 1.  Hybridization histochemistry.

Authors:  J D Penschow; J Haralambidis; P E Darling; I A Darby; E M Wintour; G W Tregear; J P Coghlan
Journal:  Experientia       Date:  1987-07-15

2.  Localization of enkephalins in adrenaline cells and the nerves innervating adrenaline cells in rat adrenal medulla.

Authors:  M Pelto-Huikko; T Salminen; A Hervonen
Journal:  Histochemistry       Date:  1985

3.  In situ hybridization using 32P labelled oligodeoxyribonucleotides for the cellular localisation of mRNA in neuronal and endocrine tissue. An analysis of procedural variables.

Authors:  J V Priestley; M A Hynes; V K Han; M Réthelyi; E R Perl; P K Lund
Journal:  Histochemistry       Date:  1988

4.  Transferrin gene expression visualized in oligodendrocytes of the rat brain by using in situ hybridization and immunohistochemistry.

Authors:  B Bloch; T Popovici; M J Levin; D Tuil; A Kahn
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

5.  Detection of proopiomelanocortin mRNA by in situ hybridization with an oligonucleotide probe.

Authors:  M E Lewis; T G Sherman; S Burke; H Akil; L G Davis; R Arentzen; S J Watson
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

6.  Nerve growth factor mRNA and protein in the testis and epididymis of mouse and rat.

Authors:  C Ayer-LeLievre; L Olson; T Ebendal; F Hallböök; H Persson
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

7.  Detection of nerve growth factor and its mRNA by separate and combined immunohistochemistry and in situ hybridization in mouse salivary glands.

Authors:  C Ayer-Le Lievre; T Ebendal; L Olson; A Seiger; H Persson
Journal:  Histochem J       Date:  1989-01

8.  Quantitation of proenkephalin A messenger RNA in bovine brain, pituitary and adrenal medulla: correlation between mRNA and peptide levels.

Authors:  C W Pittius; N Kley; J P Loeffler; V Höllt
Journal:  EMBO J       Date:  1985-05       Impact factor: 11.598
  8 in total

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