Literature DB >> 6546810

A pure enzyme catalyzing penicillin biosynthesis.

I J Hollander, Y Q Shen, J Heim, A L Demain, S Wolfe.   

Abstract

Isopenicillin N synthetase (cyclase) has been purified to homogeneity from Cephalosporium acremonium strain C-10. The enzyme has a molecular weight of 40,000 to 42,000 and yields a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme was purified in 10 percent yield by a combination of protamine sulfate and ammonium sulfate precipitations, gel filtration, and ion-exchange high-performance liquid chromatography. The purified enzyme can be stabilized with sucrose and stored at -20 degrees C for several weeks without any loss in activity.

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Year:  1984        PMID： 6546810     DOI： 10.1126/science.6546810

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  14 in total

Review 1.  Enzyme stabilization: state of the art.

Authors:  L Gianfreda; M R Scarfi
Journal:  Mol Cell Biochem       Date:  1991-02-02       Impact factor: 3.396

Review 2.  Nonribosomal peptide synthetases involved in the production of medically relevant natural products.

Authors:  Elizabeth A Felnagle; Emily E Jackson; Yolande A Chan; Angela M Podevels; Andrew D Berti; Matthew D McMahon; Michael G Thomas
Journal:  Mol Pharm       Date:  2008-01-25       Impact factor: 4.939

Review 3.  Enzymes and aflatoxin biosynthesis.

Authors:  M F Dutton
Journal:  Microbiol Rev       Date:  1988-06

4.  Purification of isopenicillin N synthetase.

Authors:  C P Pang; B Chakravarti; R M Adlington; H H Ting; R L White; G S Jayatilake; J E Baldwin; E P Abraham
Journal:  Biochem J       Date:  1984-09-15       Impact factor: 3.857

Review 5.  Molecular regulation of beta-lactam biosynthesis in filamentous fungi.

Authors:  A A Brakhage
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

6.  Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium.

Authors:  J E Dotzlaf; W K Yeh
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

7.  Expression of fungal genes involved in penicllin biosynthesis.

Authors:  M A Peñalva; E Espeso; B Pérez-Esteban; M Orejas; J M Fernández-Cañón; H Martínez-Blanco
Journal:  World J Microbiol Biotechnol       Date:  1993-07       Impact factor: 3.312

8.  Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Authors:  J E Baldwin; R M Adlington; J B Coates; M J Crabbe; N P Crouch; J W Keeping; G C Knight; C J Schofield; H H Ting; C A Vallejo
Journal:  Biochem J       Date:  1987-08-01       Impact factor: 3.857

9.  Isopenicillin N synthetase of Penicillium chrysogenum, an enzyme that converts delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine to isopenicillin N.

Authors:  F R Ramos; M J López-Nieto; J F Martín
Journal:  Antimicrob Agents Chemother       Date:  1985-03       Impact factor: 5.191

10.  Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase.

Authors:  S Kovacevic; B J Weigel; M B Tobin; T D Ingolia; J R Miller
Journal:  J Bacteriol       Date:  1989-02       Impact factor: 3.490
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